Evidence map›Paper›PMID 27987021›Full record

ArticleInternational urogynecology journal2017

Impaired contraction and decreased detrusor innervation in a female rat model of pelvic neuropraxia.

Johanna L Hannan, Shelby A Powers, Vinson M Wang, Fabio Castiglione, Petter Hedlund, Trinity J Bivalacqua

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International urogynecology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.4field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

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  7. Pathophysiology of the underactive bladder.Investigative and clinical urology · 2017
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 4 countries.

Johanna L HannanDepartment of Physiology, Brody School of Medicine, East Carolina University, 600 Moye Boulevard, Mail Stop 634, Greenville, NC, 27834-4354, USA. hannanj14@ecu.edu.
Shelby A PowersDepartment of Physiology, Brody School of Medicine, East Carolina University, 600 Moye Boulevard, Mail Stop 634, Greenville, NC, 27834-4354, USA.
Vinson M WangThe James Buchanan Brady Urological Institute, and Department of Urology, The Johns Hopkins School of Medicine, 600 North Wolfe Street, Marburg 420, Baltimore, MD, 21287, USA.
Fabio CastiglioneUnit of Urology, Division of Oncology, Urological Research Institute, IRCCS Ospedale San Raffaele, Milan, Italy.
Petter HedlundUnit of Urology, Division of Oncology, Urological Research Institute, IRCCS Ospedale San Raffaele, Milan, Italy.
Trinity J BivalacquaThe James Buchanan Brady Urological Institute, and Department of Urology, The Johns Hopkins School of Medicine, 600 North Wolfe Street, Marburg 420, Baltimore, MD, 21287, USA.
East Carolina University · USJohns Hopkins University · USKU Leuven · BELund University · SE

Funding

Roles of RhoA/Rho-kinase Signaling in Cavernous Neurons Following Axonal InjuryK08DK090370 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI BIVALACQUA, TRINITY JUDE · 2011 to 2015
$794k
NIDDK NIH HHS K08 DK090370
6 · The paper itself

Abstract

introduction and hypothesisBilateral pelvic nerve injury (BPNI) is a model of post-radical hysterectomy neuropraxia, a common sequela. This study assessed the time course of changes to detrusor autonomic innervation, smooth muscle (SM) content and cholinergic-mediated contraction post-BPNI.

methodsFemale Sprague-Dawley rats underwent BPNI or sham surgery and were evaluated 3, 7, 14, and 30 days post-BPNI (n = 8/group). Electrical field-stimulated (EFS) and carbachol-induced contractions were measured. Gene expression was assessed by qPCR for muscarinic receptor types 2 (M2) and 3 (M3), collagen type 1α1 and 3α1, and SM actin. Western blots measured M2 and M3 protein expression. Bladder sections were stained with Masson's trichrome for SM content and immunofluorescence staining for nerve terminals expressing vesicular acetylcholine transporter (VAChT), tyrosine hydroxylase (TH), and neuronal nitric oxide synthase (nNOS).

resultsBilateral pelvic nerve injury caused larger bladders with less SM content and increased collagen type 1α1 and 3α1 gene expression. At early time points, cholinergic-mediated contraction increased, whereas EFS-mediated contraction decreased and returned to baseline by 30 days. Protein and gene expression of M3 was decreased 3 and 7 days post-BPNI, whereas M2 was unchanged. TH nerve terminals surrounding the detrusor decreased in all BPNI groups, whereas VAChT and nNOS terminals decreased 14 and 30 days post-BPNI.

conclusionsBilateral pelvic nerve injury increased bladder size, impaired contractility, and decreased SM and autonomic innervation. Therapeutic strategies preventing nerve injury-mediated decline in neuronal input and SM content may prevent the development of a neurogenic bladder and improve quality of life after invasive pelvic surgery.

Indexed as

ActinsAnimalsCollagenDisease Models, AnimalElectric StimulationFemaleHysterectomyMuscarinic AgonistsPeripheral Nerve InjuriesRats, Sprague-DawleyReceptors, MuscarinicUrinary BladderActa2 protein, ratActinsCollagenMuscarinic AgonistsReceptors, MuscarinicAutonomic innervationDetrusorNeurogenic bladderNeuropraxiaRadical hysterectomy

Identifiers

PMID27987021
PMCPMC5474207
OpenAlexW2564912877

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.