Evidence map›Paper›PMID 27986867›Full record

ArticlePlant physiology2017

Sucrose Transporter Localization and Function in Phloem Unloading in Developing Stems.

Ricky J Milne, Jai M Perroux, Anne L Rae, Anke Reinders, John M Ward, Christina E Offler, John W Patrick, Christopher P L Grof

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
15.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 86 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Plant direct · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Microorganisms · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Ricky J MilneSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (R.J.M., C.E.O., J.W.P., C.P.L.G.).ORCID 0000-0002-9792-4175
Jai M PerrouxSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (R.J.M., C.E.O., J.W.P., C.P.L.G.).ORCID 0000-0003-0765-4026
Anne L RaeSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (R.J.M., C.E.O., J.W.P., C.P.L.G.).
Anke ReindersSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (R.J.M., C.E.O., J.W.P., C.P.L.G.).
John M WardSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (R.J.M., C.E.O., J.W.P., C.P.L.G.).ORCID 0000-0001-8185-0434
Christina E OfflerSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (R.J.M., C.E.O., J.W.P., C.P.L.G.).ORCID 0000-0002-5403-4834
John W PatrickSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (R.J.M., C.E.O., J.W.P., C.P.L.G.).ORCID 0000-0003-0964-9327
Christopher P L GrofSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia (R.J.M., C.E.O., J.W.P., C.P.L.G.); chris.grof@newcastle.edu.au.ORCID 0000-0003-1968-5401
Commonwealth Scientific and Industrial Research Organisation · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

How sucrose transporters (SUTs) regulate phloem unloading in monocot stems is poorly understood and particularly so for species storing high Suc concentrations. To this end, Sorghum bicolor SUTs SbSUT1 and SbSUT5 were characterized by determining their transport properties heterologously expressed in yeast or Xenopus laevis oocytes, and their in planta cellular and subcellular localization. The plasma membrane-localized SbSUT1 and SbSUT5 exhibited a strong selectivity for Suc and high Suc affinities in X. laevis oocytes at pH 5-SbSUT1, 6.3 ± 0.7 mm, and SbSUT5, 2.4 ± 0.5 mm Suc. The Suc affinity of SbSUT1 was dependent on membrane potential and pH. In contrast, SbSUT5 Suc affinity was independent of membrane potential and pH but supported high transport rates at neutral pH. Suc transport by the tonoplast localized SbSUT4 could not be detected using yeast or X. laevis oocytes. Across internode development, SUTs, other than SbSUT4, were immunolocalized to sieve elements, while for elongating and recently elongated internodes, SUTs also were detected in storage parenchyma cells. We conclude that apoplasmic Suc unloading from de-energized protophloem sieve elements in meristematic zones may be mediated by reversal of SbSUT1 and/or by uniporting SWEETs. Storage parenchyma localized SbSUT1 and SbSUT5 may accumulate Suc from the stem apoplasms of elongating and recently elongated internodes, whereas SbSUT4 may function to release Suc from vacuoles. Transiting from an apoplasmic to symplasmic unloading pathway as the stem matures, SbSUT1 and SbSUT5 increasingly function in Suc retrieval into metaphloem sieve elements to maintain a high turgor to drive symplasmic unloading by bulk flow.

Indexed as

AnimalsCell MembraneGene Expression Regulation, PlantOocytesPhloemPlant ProteinsPlant StemsSorghumSucroseXenopus laevisPlant ProteinsSucrose

Identifiers

PMID27986867
PMCPMC5291036
OpenAlexW2565385908

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.