ArticleNucleic acids research2017
Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Systematic Review of Plant Ribosome Heterogeneity and Specialization.Frontiers in plant science · 2020Pooled it
- Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome.Nature plants · 2026Article
- Rapid plastid isolation reveals the chloroplast proteome and structures of the chlororibosome large subunit and RuBisCO inbioRxiv : the preprint server for biology · 2026Article
- Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome.bioRxiv : the preprint server for biology · 2026Article
- Evolutionary Flexibility of Ribosome Biogenesis in Bacteria.Molecular biology and evolution · 2025Article
- Chloroplast Hibernation-Promoting Factor PSRP1 Prevents Ribosome Degradation Under Darkness Independently of 100S Dimer Formation.Plants (Basel, Switzerland) · 2025Article
- Plant MutS2 proteins function in plastid ribosome quality control.bioRxiv : the preprint server for biology · 2025Article
- Defective Processing of Cytoplasmic and Chloroplast Ribosomal RNA in the Absence of Arabidopsis DXO1.Plant, cell & environment · 2025Article
- The uS10c-BPG2 module mediates ribosomal RNA processing in chloroplast nucleoids.Nucleic acids research · 2024Article
- High-Resolution Structure and Internal Mobility of a Plant 40S Ribosomal Subunit.International journal of molecular sciences · 2023Article
- Perturbation of protein homeostasis brings plastids at the crossroad between repair and dismantling.PLoS genetics · 2023Article
- Types and Functions of Mitoribosome-Specific Ribosomal Proteins across Eukaryotes.International journal of molecular sciences · 2022Review
- AtRsmD Is Required for Chloroplast Development and Chloroplast Function inFrontiers in plant science · 2022Article
- Stromal Protein Chloroplast Development and Biogenesis1 Is Essential for Chloroplast Development and Biogenesis inFrontiers in plant science · 2022Article
- An Unexpectedly Complex Mitoribosome in Andalucia godoyi, a Protist with the Most Bacteria-like Mitochondrial Genome.Molecular biology and evolution · 2021Article
- Arabidopsis mTERF9 protein promotes chloroplast ribosomal assembly and translation by establishing ribonucleoprotein interactions in vivo.Nucleic acids research · 2021Article
- The Role of Chloroplast Gene Expression in Plant Responses to Environmental Stress.International journal of molecular sciences · 2020Review
- The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development.Nucleic acids research · 2020Article
- The Arabidopsis Protein CGL20 Is Required for Plastid 50S Ribosome Biogenesis.Plant physiology · 2020Article
- Co-Translational Protein Folding and Sorting in Chloroplasts.Plants (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribosomes are the protein synthesizing machines of the cell. Recent advances in cryo-EM have led to the determination of structures from a variety of species, including bacterial 70S and eukaryotic 80S ribosomes as well as mitoribosomes from eukaryotic mitochondria, however, to date high resolution structures of plastid 70S ribosomes have been lacking. Here we present a cryo-EM structure of the spinach chloroplast 70S ribosome, with an average resolution of 5.4 Å for the small 30S subunit and 3.6 Å for the large 50S ribosomal subunit. The structure reveals the location of the plastid-specific ribosomal proteins (RPs) PSRP1, PSRP4, PSRP5 and PSRP6 as well as the numerous plastid-specific extensions of the RPs. We discover many features by which the plastid-specific extensions stabilize the ribosome via establishing additional interactions with surrounding ribosomal RNA and RPs. Moreover, we identify a large conglomerate of plastid-specific protein mass adjacent to the tunnel exit site that could facilitate interaction of the chloroplast ribosome with the thylakoid membrane and the protein-targeting machinery. Comparing the Escherichia coli 70S ribosome with that of the spinach chloroplast ribosome provides detailed insight into the co-evolution of RP and rRNA.
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