ArticleApplied and environmental microbiology2017
FlrA Represses Transcription of the Biofilm-Associated bpfA Operon in Shewanella putrefaciens.
Article in Applied and environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 32 citations in OpenAlex.
- Ampicillin promotes the biofilm formation ofMicrobiology spectrum · 2026Article
- Local c-di-GMP signaling, triggered by cross-regulation of cAMP-CRP and c-di-GMP, controls biofilm formation under nutrient limitation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Investigation into the Antibacterial Mechanism of Biogenic Tellurium Nanoparticles and Precursor Tellurite.International journal of molecular sciences · 2022Article
- GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP.NPJ biofilms and microbiomes · 2022Article
- Genomic Analysis of Two Representative Strains ofFoods (Basel, Switzerland) · 2022Article
- cAMP and c-di-GMP synergistically support biofilm maintenance through the direct interaction of their effectors.Nature communications · 2022Article
- The enhanced expression of genes encoding diguanylate cyclases under cold stress contributes to the adhesion and biofilm formation ofFrontiers in nutrition · 2022Article
- YgfY Contributes to Stress Tolerance inMicroorganisms · 2021Article
- Identification of a Diguanylate Cyclase That Facilitates Biofilm Formation on Electrodes by Shewanella oneidensis MR-1.Applied and environmental microbiology · 2021Article
- Biofilm Biology and Engineering ofFrontiers in bioengineering and biotechnology · 2021Review
- The phosphorylated regulator of chemotaxis is crucial throughout biofilm biogenesis in Shewanella oneidensis.NPJ biofilms and microbiomes · 2020Article
- The GGDEF Domain of the Phosphodiesterase PdeB inJournal of bacteriology · 2019Article
- Partially Reciprocal Replacement of FlrA and FlrC in Regulation of Shewanella oneidensis Flagellar Biosynthesis.Journal of bacteriology · 2018Article
- Sodium Lactate Negatively Regulates Shewanella putrefaciens CN32 Biofilm Formation via a Three-Component Regulatory System (LrbS-LrbA-LrbR).Applied and environmental microbiology · 2017Article
- Mechanism and Role of Globin-Coupled Sensor Signalling.Advances in microbial physiology · 2017Review
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Manipulation of biofilm formation in Shewanella is beneficial for application to industrial and environmental biotechnology. BpfA is an adhesin largely responsible for biofilm formation in many Shewanella species. However, the mechanism underlying BpfA production and the resulting biofilm remains vaguely understood. We previously described the finding that BpfA expression is enhanced by DosD, an oxygen-stimulated diguanylate cyclase, under aerobic growth. In the present work, we identify FlrA as a critical transcription regulator of the bpfA operon in Shewanella putrefaciens CN32 by transposon mutagenesis. FlrA acted as a repressor of the operon promoter by binding to two boxes overlapping the -10 and -35 sites recognized by σ IMPORTANCE: Motility and biofilm are mutually exclusive lifestyles, shifts between which are under the strict regulation of bacteria attempting to adapt to the fluctuation of diverse environmental conditions. The FlrA protein in many bacteria is known to control motility as a master regulator of flagellum synthesis. This work elucidates its effect on biofilm formation by controlling the expression of the adhesin BpfA in S. putrefaciens CN32 in response to c-di-GMP. Therefore, FlrA plays a dual role in controlling motility and biofilm formation in S. putrefaciens CN32. The cooccurrence of flrA, bpfA, and the FlrA box in the promoter region of the bpfA operon in diverse Shewanella strains suggests that bpfA is a common mechanism that controls biofilm formation in this bacterial species.
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