ArticleCell2016
Single-Molecule Real-Time 3D Imaging of the Transcription Cycle by Modulation Interferometry.
Article in Cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The displacement of the σ70 finger in initial transcription is highly heterogeneous and promoter-dependent.Nucleic acids research · 2025Article
- Single-protein/RNA imaging reveals ZNF598 as a limiting factor in resolving collided ribosomes.The EMBO journal · 2025Article
- RNAP Promoter Search and Transcription Kinetics in LiveThe journal of physical chemistry. B · 2023Article
- Mechanisms of transcription control by distal enhancers from high-resolution single-gene imaging.bioRxiv : the preprint server for biology · 2023Article
- Direct-laser writing for subnanometer focusing and single-molecule imaging.Nature communications · 2022Article
- Imaging the endocannabinoid signaling system.Journal of neuroscience methods · 2022Review
- New insights into promoter-enhancer communication mechanisms revealed by dynamic single-molecule imaging.Biochemical Society transactions · 2021Review
- Simple and versatile imaging of genomic loci in live mammalian cells and early pre-implantation embryos using CAS-LiveFISH.Scientific reports · 2021Article
- A Single-Molecule Surface-Based Platform to Detect the Assembly and Function of the Human RNA Polymerase II Transcription Machinery.Structure (London, England : 1993) · 2020Article
- Single-gene imaging links genome topology, promoter-enhancer communication and transcription control.Nature structural & molecular biology · 2020Article
- Real-time 3D single molecule tracking.Nature communications · 2020Article
- Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells.Cell · 2019Article
- Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts.Nature communications · 2018Article
- Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.Scientific reports · 2018Article
- Effects of mRNA Degradation and Site-Specific Transcriptional Pausing on Protein Expression Noise.Biophysical journal · 2018Article
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
- Studying transcription initiation by RNA polymerase with diffusion-based single-molecule fluorescence.Protein science : a publication of the Protein Society · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Many essential cellular processes, such as gene control, employ elaborate mechanisms involving the coordination of large, multi-component molecular assemblies. Few structural biology tools presently have the combined spatial-temporal resolution and molecular specificity required to capture the movement, conformational changes, and subunit association-dissociation kinetics, three fundamental elements of how such intricate molecular machines work. Here, we report a 3D single-molecule super-resolution imaging study using modulation interferometry and phase-sensitive detection that achieves <2 nm axial localization precision, well below the few-nanometer-sized individual protein components. To illustrate the capability of this technique in probing the dynamics of complex macromolecular machines, we visualize the movement of individual multi-subunit E. coli RNA polymerases through the complete transcription cycle, dissect the kinetics of the initiation-elongation transition, and determine the fate of σ
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.