ArticleJournal of immunoassay & immunochemistry2017
A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays.
Article in Journal of immunoassay & immunochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
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- Enhanced sensitivity of lateral flow assay for NGAL detection using cysteamine-modified gold nanoparticles.RSC advances · 2025Article
- Development of Novel Neratinib and Docetaxel Core-Loaded and Trastuzumab Surface-Conjugated Nanoparticle for Treatment of HER-2 Positive Breast Cancer.Pharmaceutics · 2025Article
- Design Principles for Engineering Ionic Liquid-Gold Nanoparticles for Therapeutic Delivery to the Brain.ACS nano · 2025Article
- Proteomics-Empowered Microfluidic-SERS Immunoassay for Identifying and Detecting Biomarkers of Micropapillary Lung Adenocarcinoma.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Active targeting of type 1 diabetes therapies to pancreatic beta cells using nanocarriers.Diabetologia · 2025Review
- Plasmonic silver and gold nanoparticles: shape- and structure-modulated plasmonic functionality for point-of-caring sensing, bio-imaging and medical therapy.Chemical Society reviews · 2024Review
- Dual conjugation of magnetic nanoparticles with antibodies and siRNA for cell-specific gene silencing in vascular cells.Frontiers in drug delivery · 2024Article
- Antibody-labelled gold nanoparticles synthesized by laser ablation to detect SARS-CoV-2 antigen spike.ADMET & DMPK · 2024Article
- pH and NaCl Optimisation to Improve the Stability of Gold and Silver Nanoparticles' Anti-Zearalenone Antibody Conjugates for Immunochromatographic Assay.Methods and protocols · 2023Article
- Gold Nanoparticle Paper Immunoassays for Sensing the Presence ofACS omega · 2023Article
- Magnetic Nanoparticles for Protein Separation and Purification.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Biophysical and biochemical insights in the design of immunoassays.Biochimica et biophysica acta. General subjects · 2023Review
- Development of an on-site lateral flow immune assay based on mango leaf derived colloidal silver nanoparticles for rapid detection ofJournal of food science and technology · 2023Article
- The Influence of Preforming Protein Coronas on the Performance of Dengue NS1 Immunoassays.Pharmaceutics · 2022Article
- Nanomaterial and interface advances in immunoassay biosensors.The journal of physical chemistry. C, Nanomaterials and interfaces · 2022Article
- Gold-based nanoplatform for a rapid lateral flow immunochromatographic test assay for gluten detection.BMC biomedical engineering · 2022Article
- High-Sensitive Detection and Quantitative Analysis of Thyroid-Stimulating Hormone Using Gold-Nanoshell-Based Lateral Flow Immunoassay Device.Biosensors · 2022Article
- Gold nanoparticles in virus detection: Recent advances and potential considerations for SARS-CoV-2 testing development.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
- Point-of-Care Devices to Detect Zika and Other Emerging Viruses.Annual review of biomedical engineering · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips. Results show that orientational, covalent conjugation with PEG shield could improve NS1 detection. These approaches can be used to optimize immunochromatographic detection for a range of biomarkers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.