Evidence map›Paper›PMID 27982129›Full record

ArticleScientific reports2016

Phosphorylation of SKAP by GSK3β ensures chromosome segregation by a temporal inhibition of Kif2b activity.

Bo Qin, Dan Cao, Huihui Wu, Fei Mo, Hengyi Shao, Jane Chu, Michael Powell, Felix Aikhionbare, Dongmei Wang, Chuanhai Fu and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 2 countries.

Bo QinAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Dan CaoAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Huihui WuAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Fei MoAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Hengyi ShaoAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Jane ChuMolecular Imaging Center, Atlanta Clinical &Translational Science Institute, Atlanta, GA 30310.
Michael PowellMolecular Imaging Center, Atlanta Clinical &Translational Science Institute, Atlanta, GA 30310.
Felix AikhionbareMolecular Imaging Center, Atlanta Clinical &Translational Science Institute, Atlanta, GA 30310.
Dongmei WangAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Chuanhai FuAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Ping HeGuangzhou Women and Children's Medical Center, Guangzhou 510623, China.
Weijun PanShanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Wenwen WangAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Xing LiuAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
Xuebiao YaoAnhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science &Technology of China, Hefei 230027, China.
University of Science and Technology of China · CNImaging Center · USCenter for Excellence in Molecular Cell Science · CNChinese Academy of Sciences · CNGuangzhou Medical University · CNShanghai Institutes for Biological Sciences · CN

Funding

The function of ezrin in stimulus-coupled acid secretionR01DK056292 · NIDDK · UNIVERSITY OF WISCONSIN MADISON · PI YAO, XUEBIAO · 1999 to 2012
$2.5M
Function of ACAP4 in CCL18-stimulated breast cancer metastasisR01CA164133 · NCI · MOREHOUSE SCHOOL OF MEDICINE · PI YAO, XUEBIAO · 2012 to 2016
$1.4M
NCI NIH HHS R01 CA164133NIDDK NIH HHS R01 DK056292
6 · The paper itself

Abstract

Chromosome segregation in mitosis is orchestrated by the dynamic interactions between the kinetochore and spindle microtubules. Our recent study shows SKAP is an EB1-dependent, microtubule plus-end tracking protein essential for kinetochore oscillations during mitosis. Here we show that phosphorylation of SKAP by GSK3β regulates Kif2b depolymerase activity by competing Kif2b for microtubule plus-end binding. SKAP is a bona fide substrate of GSK3β in vitro and the phosphorylation is essential for an accurate kinetochore-microtubule attachment in cells. The GSK3β-elicited phosphorylation sites were mapped by mass spectrometry and the phosphomimetic mutant of SKAP can rescue the phenotype of chromosome missegregation in SKAP-suppressed cells. Importantly, GSK3β-elicited phosphorylation promotes SKAP binding to Kif2b to regulate its depolymerase activity at the microtubule plus-ends. Based on those findings, we reason that GSK3β-SKAP-Kif2b signaling axis constitutes a dynamic link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division.

Indexed as

Cell Cycle ProteinsChromosome SegregationChromosomes, HumanGlycogen Synthase Kinase 3 betaHeLa CellsHumansKinesinsMicrotubule-Associated ProteinsPhosphorylationSignal TransductionCell Cycle ProteinsGlycogen Synthase Kinase 3 betaGSK3B protein, humanKIF2B protein, humanKinesinsKNSTRN protein, humanMicrotubule-Associated Proteins

Identifiers

PMID27982129
PMCPMC5159797
OpenAlexW2561261294

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.