Evidence map›Paper›PMID 27982116›Full record

ArticleScientific reports2016

A method for high-throughput functional imaging of single cells within heterogeneous cell preparations.

Adam S Neal, Austin M Rountree, Jared R Radtke, Jianzhu Yin, Michael W Schwartz, Christiane S Hampe, Jonathan D Posner, Vincenzo Cirulli, Ian R Sweet

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Adam S NealUW Diabetes Institute, Department of Medicine, University of Washington, Seattle, WA, 98195, USA.
Austin M RountreeUW Diabetes Institute, Department of Medicine, University of Washington, Seattle, WA, 98195, USA.
Jared R RadtkeUW Diabetes Institute, Department of Medicine, University of Washington, Seattle, WA, 98195, USA.
Jianzhu YinDepartment of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.
Michael W SchwartzUW Diabetes Institute, Department of Medicine, University of Washington, Seattle, WA, 98195, USA.
Christiane S HampeUW Diabetes Institute, Department of Medicine, University of Washington, Seattle, WA, 98195, USA.
Jonathan D PosnerDepartment of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.
Vincenzo CirulliUW Diabetes Institute, Department of Medicine, University of Washington, Seattle, WA, 98195, USA.
Ian R SweetUW Diabetes Institute, Department of Medicine, University of Washington, Seattle, WA, 98195, USA.

Funding

Vector and Transgenic Mouse CoreP30DK017047 · NIDDK · UNIVERSITY OF WASHINGTON · PI Karin E Bornfeldt · 1986 to 2026
$41.4M
Novel Brain Mechanisms Controlling Glucose HomeostasisR01DK083042 · NIDDK · UNIVERSITY OF WASHINGTON · PI SCHWARTZ, MICHAEL W · 2008 to 2025
$7.6M
ISLET CELL MEMBRANE ANTIBODIES IN DIABTESR01DK026190 · NIDDK · UNIVERSITY OF WASHINGTON · PI HAMPE, CHRISTIANE S · 1986 to 2016
$4.9M
Novel Anti-Diabetic Actions of Hypothalamic FGF19-FGFR1 SignalingR01DK101997 · NIDDK · UNIVERSITY OF WASHINGTON · PI SCHWARTZ, MICHAEL W · 2014 to 2018
$1.9M
Continuous measurement of cell growth as an optimal tool in drug toxicity testing (Supplement)R41TR001196 · NCATS · ENTOX SCIENCES, LLC · PI COOK, DANIEL L · 2015 to 2016
$274k
NCATS NIH HHS R41 TR001196NIDDK NIH HHS P30 DK017047NIDDK NIH HHS R01 DK026190NIDDK NIH HHS R01 DK083042NIDDK NIH HHS R01 DK101997
6 · The paper itself

Abstract

Functional characterization of individual cells within heterogeneous tissue preparations is challenging. Here, we report the development of a versatile imaging method that assesses single cell responses of various endpoints in real time, while identifying the individual cell types. Endpoints that can be measured include (but are not limited to) ionic flux (calcium, sodium, potassium and hydrogen), metabolic responsiveness (NAD(P)H, mitochondrial membrane potential), and signal transduction (H

Indexed as

AnimalsGlucagon-Secreting CellsGlucoseHigh-Throughput Screening AssaysImmunohistochemistryInsulin-Secreting CellsMaleMicroscopy, FluorescenceNADPOptical ImagingRats, Sprague-DawleySingle-Cell AnalysisGlucoseNADP

Identifiers

PMID27982116
PMCPMC5159830

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.