ArticleScientific reports2016
Generation of kidney tubular organoids from human pluripotent stem cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Advances and future perspectives of kidney organoid technology in renal disease research and clinical translation.Frontiers in medicine · 2026Review
- KCNJ16-depleted kidney organoids recapitulate tubulopathy and lipid recovery upon statins treatment.Stem cell research & therapy · 2024Article
- Autophagic Regulation of Adipogenesis Through TP53INP2: Insights from In Silico and In Vitro Analysis.Molecular biotechnology · 2024Article
- Extracellular Microenvironmental Control for Organoid Assembly.Tissue engineering. Part B, Reviews · 2022Review
- Urological cancer organoids, patients' avatars for precision medicine: past, present and future.Cell & bioscience · 2022Review
- Multidisciplinary approaches for elucidating genetics and molecular pathogenesis of urinary tract malformations.Kidney international · 2022Review
- Bioengineered Kidney Models: Methods and Functional Assessments.Function (Oxford, England) · 2021Review
- Baicalin reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal injury in type 1 diabetic mouse model.Cell cycle (Georgetown, Tex.) · 2020Article
- The emergence of regenerative medicine in organ transplantation: 1st European Cell Therapy and Organ Regeneration Section meeting.Transplant international : official journal of the European Society for Organ Transplantation · 2020Review
- Formation and optimization of three-dimensional organoids generated from urine-derived stem cells for renal function in vitro.Stem cell research & therapy · 2020Article
- Kidney organoids in translational medicine: Disease modeling and regenerative medicine.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Review
- In Vivo Developmental Trajectories of Human Podocyte Inform In Vitro Differentiation of Pluripotent Stem Cell-Derived Podocytes.Developmental cell · 2019Article
- Elastography of multicellular spheroids using 3D light microscopy.Biomedical optics express · 2019Article
- Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis.Sensors (Basel, Switzerland) · 2019Article
- Modelling Duchenne muscular dystrophy in MYOD1-converted urine-derived cells treated with 3-deazaneplanocin A hydrochloride.Scientific reports · 2019Article
- Organoids as a new model for improving regenerative medicine and cancer personalized therapy in renal diseases.Cell death & disease · 2019Article
- Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors.Scientific reports · 2019Article
- A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells.Stem cell reports · 2018Article
- CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.Current protocols in stem cell biology · 2018Article
- Concise Review: Kidney Generation with Human Pluripotent Stem Cells.Stem cells (Dayton, Ohio) · 2017Review
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in stem cell research have resulted in methods to generate kidney organoids from human pluripotent stem cells (hPSCs), which contain cells of multiple lineages including nephron epithelial cells. Methods to purify specific types of cells from differentiated hPSCs, however, have not been established well. For bioengineering, cell transplantation, and disease modeling, it would be useful to establish those methods to obtain pure populations of specific types of kidney cells. Here, we report a simple two-step differentiation protocol to generate kidney tubular organoids from hPSCs with direct purification of KSP (kidney specific protein)-positive cells using anti-KSP antibody. We first differentiated hPSCs into mesoderm cells using a glycogen synthase kinase-3β inhibitor for 3 days, then cultured cells in renal epithelial growth medium to induce KSP+ cells. We purified KSP+ cells using flow cytometry with anti-KSP antibody, which exhibited characteristics of all segments of kidney tubular cells and cultured KSP+ cells in 3D Matrigel, which formed tubular organoids in vitro. The formation of tubular organoids by KSP+ cells induced the acquisition of functional kidney tubules. KSP+ cells also allowed for the generation of chimeric kidney cultures in which human cells self-assembled into 3D tubular structures in combination with mouse embryonic kidney cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.