ArticleNature communications2016
Engineering orthogonal dual transcription factors for multi-input synthetic promoters.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Do plasmid-dependent phages enable the survival of costly plasmids?bioRxiv : the preprint server for biology · 2026Article
- Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system.Nature microbiology · 2026Article
- Dynamics of genetic circuits in Pseudomonas protegens.Cell systems · 2026Article
- CF2H: a cell-free two-hybrid platform for rapid protein binder screening.Nature communications · 2026Article
- Development of Metabolite-Responsive Transcription Factor Systems as Modular Platforms for Gene Expression Control.Biosensors · 2025Article
- Why cellular computations challenge our design principles.Seminars in cell & developmental biology · 2025Review
- Rapid discovery of cyclic peptide protein aggregation inhibitors by continuous selection.Nature chemical biology · 2025Article
- Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia.Nucleic acids research · 2025Article
- Regulatory Components for Bacterial Cell-Free Systems Engineering.ACS synthetic biology · 2024Review
- Synthetic gene circuit evolution: Insights and opportunities at the mid-scale.Cell chemical biology · 2024Review
- Engineering Tunable, Low Latency Spatial Computation with Dual Input Quorum Sensing Promoters.ACS synthetic biology · 2024Article
- T7 phage-assisted evolution of riboswitches using error-prone replication and dual selection.Scientific reports · 2024Article
- Accelerating Genetic Sensor Development, Scale-up, and Deployment Using Synthetic Biology.Biodesign research · 2024Article
- Development of new binary expression systems for plant synthetic biology.Plant cell reports · 2023Article
- A primer to directed evolution: current methodologies and future directions.RSC chemical biology · 2023Review
- A hierarchy of biomolecular proportional-integral-derivative feedback controllers for robust perfect adaptation and dynamic performance.Nature communications · 2022Article
- The transcription activator AtxA fromSynthetic biology (Oxford, England) · 2022Article
- Phage satellites and their emerging applications in biotechnology.FEMS microbiology reviews · 2021Review
- Engineering of a Promoter Repressed by a Light-Regulated Transcription Factor inBiodesign research · 2021Article
- Phage-assisted continuous and non-continuous evolution.Nature protocols · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Synthetic biology has seen an explosive growth in the capability of engineering artificial gene circuits from transcription factors (TFs), particularly in bacteria. However, most artificial networks still employ the same core set of TFs (for example LacI, TetR and cI). The TFs mostly function via repression and it is difficult to integrate multiple inputs in promoter logic. Here we present to our knowledge the first set of dual activator-repressor switches for orthogonal logic gates, based on bacteriophage λ cI variants and multi-input promoter architectures. Our toolkit contains 12 TFs, flexibly operating as activators, repressors, dual activator-repressors or dual repressor-repressors, on up to 270 synthetic promoters. To engineer non cross-reacting cI variants, we design a new M13 phagemid-based system for the directed evolution of biomolecules. Because cI is used in so many synthetic biology projects, the new set of variants will easily slot into the existing projects of other groups, greatly expanding current engineering capacities.
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Registered trials
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