Evidence map›Paper›PMID 27981750›Full record

ArticleChembiochem : a European journal of chemical biology2016

Isomeric Detergent Comparison for Membrane Protein Stability: Importance of Inter-Alkyl-Chain Distance and Alkyl Chain Length.

Kyung Ho Cho, Parameswaran Hariharan, Jonas S Mortensen, Yang Du, Anne K Nielsen, Bernadette Byrne, Brian K Kobilka, Claus J Loland, Lan Guan, Pil Seok Chae

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Glyco-Steroidal Amphiphiles (GSAs) for Membrane Protein Structural Study.Chembiochem : a European journal of chemical biology · 2022
    Article
  5. Article
  6. Article
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  9. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kyung Ho ChoDepartment of Bionanotechnology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, 15588, Korea.ORCID http://orcid.org/0000-0001-6038-0816
Parameswaran HariharanDepartment of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, 3601 4thStreet MS 6551, Lubbock, TX, 79430, USA.ORCID http://orcid.org/0000-0002-6020-1547
Jonas S MortensenDepartment of Neuroscience and Pharmacology, University of Copenhagen, Blegdamsvej 3, 18.6 Panum Institute, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-6743-276X
Yang DuMolecular and Cellular Physiology, Stanford University, 157 Beckman Center, 279 Campus Drive, Stanford, CA, 94305, USA.ORCID http://orcid.org/0000-0001-5115-0736
Anne K NielsenDepartment of Neuroscience and Pharmacology, University of Copenhagen, Blegdamsvej 3, 18.6 Panum Institute, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-6627-3330
Bernadette ByrneDepartment of Life Sciences, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK.ORCID http://orcid.org/0000-0001-9598-9832
Brian K KobilkaMolecular and Cellular Physiology, Stanford University, 157 Beckman Center, 279 Campus Drive, Stanford, CA, 94305, USA.ORCID http://orcid.org/0000-0001-5958-3990
Claus J LolandDepartment of Neuroscience and Pharmacology, University of Copenhagen, Blegdamsvej 3, 18.6 Panum Institute, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-1773-1446
Lan GuanDepartment of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, 3601 4thStreet MS 6551, Lubbock, TX, 79430, USA.ORCID http://orcid.org/0000-0002-2274-361X
Pil Seok ChaeDepartment of Bionanotechnology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, 15588, Korea.ORCID http://orcid.org/0000-0003-1799-3304

Funding

THE MECHANISM OF BETA2 ADRENOCEPTOR ACTIVATIONR01NS028471 · NINDS · STANFORD UNIVERSITY · PI KOBILKA, BRIAN K · 1990 to 2021
$6.7M
NINDS NIH HHS R01 NS028471
6 · The paper itself

Abstract

Membrane proteins encapsulated by detergent micelles are widely used for structural study. Because of their amphipathic property, detergents have the ability to maintain protein solubility and stability in an aqueous medium. However, conventional detergents have serious limitations in their scope and utility, particularly for eukaryotic membrane proteins and membrane protein complexes. Thus, a number of new agents have been devised; some have made significant contributions to membrane protein structural studies. However, few detergent design principles are available. In this study, we prepared meta and ortho isomers of the previously reported para-substituted xylene-linked maltoside amphiphiles (XMAs), along with alkyl chain-length variation. The isomeric XMAs were assessed with three membrane proteins, and the meta isomer with a C

Indexed as

MicellesBacterial ProteinsDetergentsHydrophobic and Hydrophilic InteractionsIsomerismMembrane ProteinsProtein StabilitySalmonella typhimuriumSolubilitySymportersTemperatureXylenes4-xyleneBacterial ProteinsDetergentsmelibiose permeaseMembrane ProteinsMicellesSymportersXylenesamphiphilesdetergentsmembrane proteinsnoncovalent interactionsprotein solubilizationprotein stabilization

Identifiers

PMID27981750
PMCPMC5500196

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.