ArticleAngewandte Chemie (International ed. in English)2017
A Redox-Activatable Fluorescent Sensor for the High-Throughput Quantification of Cytosolic Delivery of Macromolecules.
Article in Angewandte Chemie (International ed. in English), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Review
- Magnetic Resonance Imaging-Based Quantification of Endosomal Escape Using Iron Oxide Nanoparticle-Loaded Lipid Nanoparticles.Advanced healthcare materials · 2025Article
- A Multi-Threshold Micelle Improves Tumor Accumulation and STING Immunotherapy.Journal of the American Chemical Society · 2025Article
- Near-Infrared Bioimaging Using Two-photon Fluorescent Probes.Advanced healthcare materials · 2025Review
- Engineered aptamers for molecular imaging.Chemical science · 2023Review
- Elucidation of Protonation Cooperativity of a STING-Activating Polymer.Advanced materials (Deerfield Beach, Fla.) · 2023Article
- Synthetic Proteins behind the Plasma Barrier: Molecular Spies.Accounts of chemical research · 2022Article
- Polyvalent design in the cGAS-STING pathway.Seminars in immunology · 2021Review
- Review
- Antigen folding improves loading efficiency and antitumor efficacy of PC7A nanoparticle vaccine.Journal of controlled release : official journal of the Controlled Release Society · 2021Article
- Nanoscopy for endosomal escape quantification.Nanoscale advances · 2021Review
- Nano-Immune-Engineering Approaches to Advance Cancer Immunotherapy: Lessons from Ultra-pH-Sensitive Nanoparticles.Accounts of chemical research · 2020Article
- Disulfide-containing Macromolecules for Therapeutic Delivery.Israel journal of chemistry · 2020Article
- Recent Development of pH-Responsive Polymers for Cancer Nanomedicine.Molecules (Basel, Switzerland) · 2018Review
- A sequence-activated AND logic dual-channel fluorescent probe for tracking programmable drug release.Chemical science · 2018Article
- Cooperativity Principles in Self-Assembled Nanomedicine.Chemical reviews · 2018Review
- Optical molecular imaging for tumor detection and image-guided surgery.Biomaterials · 2018Review
- Endogenous pH-responsive nanoparticles with programmable size changes for targeted tumor therapy and imaging applications.Theranostics · 2018Review
- Innate Immune Activation by cGMP-AMP Nanoparticles Leads to Potent and Long-Acting Antiretroviral Response against HIV-1.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- A STING-activating nanovaccine for cancer immunotherapy.Nature nanotechnology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Efficient delivery of biomacromolecules (e.g., proteins, nucleic acids) into cell cytosol remains a critical challenge for the development of macromolecular therapeutics or diagnostics. To date, most common approaches to assess cytosolic delivery rely on fluorescent labeling of macromolecules with an "always on" reporter and subcellular imaging of endolysosomal escape by confocal microscopy. This strategy is limited by poor signal-to-noise ratio and only offers low throughput, qualitative information. Herein we describe a quantitative redox-activatable sensor (qRAS) for the real-time monitoring of cytosolic delivery of macromolecules. qRAS-labeled macromolecules are silent (off) inside the intact endocytic organelles, but can be turned on by redox activation after endolysosomal disruption and delivery into the cytosol, thereby greatly improving the detection accuracy. In addition to confocal microscopy, this quantitative sensing technology allowed for a high-throughput screening of a panel of polymer carriers toward efficient cytosolic delivery of model proteins on a plate reader. The simple and versatile qRAS design offers a useful tool for the investigation of new strategies for endolysosomal escape of biomacromolecules to facilitate the development of macromolecular therapeutics for a variety of disease indications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.