Evidence map›Paper›PMID 27981405›Full record

ArticleJournal of fluorescence2017

Study of Fluorescent Imaging of Se (IV) in Living Cells Using a Turn-on Fluorescent Probe Based on a Rhodamine Spirolactame Derivative.

Mingming Guan, Hongyu Mi, Hui Xu, Qiang Fei, Hongyan Shan, Yanfu Huan, Shaowu Lv, Guodong Feng

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Article in Journal of fluorescence, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Mingming GuanDepartment of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Hongyu MiDepartment of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Hui XuDepartment of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Qiang FeiDepartment of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Hongyan ShanDepartment of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Yanfu HuanDepartment of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Shaowu LvKey Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, 130012, China. lvsw@jlu.edu.cn.
Guodong FengDepartment of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China. fenggd@jlu.edu.cn.
Jilin University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A highly selective fluorescent probe 2-(2-(2-aminoethylamino)ethyl)-3',6'-bis(ethylamino)-2',7'-dimethylspiro[isoindoline-1,9'-xanthen]-3-one (ABDO) for Se (IV) had been synthesized in our earlier report. In this study, this fluorescent sensor is applied on analysis fluorescent imaging of Se (IV) in Hela cells. The experiment conditions, such as the MTT assay, different concentration of saline, incubated time of Hela cells with ABDO and Se (IV), and intracellular action position of Se (IV), are investigated. Through a series of experiments, the fluorescent image of Se (IV) in Hela cells can be observed when the cells cultured by 2 μM ABDO and 2 μM Se (IV) for 210 min. And the intracellular action position of Se (IV) is verified after the co-localization experiments are done. It is mitochondria. These experimental results show that ABDO will be an eagerly anticipated sensor for fluorescent imaging analysis of selenium ion in living cells. Besides, we also can use the complexes of ABDO-Se to observe morphology and distribution of mitochondria in cells like JG-B.

Indexed as

FluorescenceFluorescent DyesHeLa CellsHumansMicroscopy, FluorescenceModels, MolecularMolecular StructureRhodaminesSelenium CompoundsFluorescent DyesRhodaminesSelenium CompoundsFluorescent probeHela cellsLive cell imagingMitochondriaRhodamineSelenium

Identifiers

PMID27981405
OpenAlexW2562818113

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.