ArticleNucleic acids research1989
Nucleotide substitutions within the cardiac troponin T alternative exon disrupt pre-mRNA alternative splicing.
Article in Nucleic acids research, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 101 citations in OpenAlex.
- mRNA Metabolism in Cardiac Development and Disease: Life After Transcription.Physiological reviews · 2020Review
- Multiple features contribute to efficient constitutive splicing of an unusually large exon.Nucleic acids research · 2001Article
- Splicing enhancement in the yeast rp51b intron.RNA (New York, N.Y.) · 2000Article
- Slow troponin T mRNA in striated muscles is expressed in both cell type and developmental stage specific manner.Journal of muscle research and cell motility · 2000Article
- Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.Molecular and cellular biology · 1999Article
- Muscle-specific splicing of a heterologous exon mediated by a single muscle-specific splicing enhancer from the cardiac troponin T gene.Molecular and cellular biology · 1998Article
- Molecular genetic dissection of mouse unconventional myosin-VA: tail region mutations.Genetics · 1998Article
- A short sequence within two purine-rich enhancers determines 5' splice site specificity.Molecular and cellular biology · 1998Article
- A naturally arising mutation of a potential silencer of exon splicing in human immunodeficiency virus type 1 induces dominant aberrant splicing and arrests virus production.Journal of virology · 1997Article
- Evolutionary conservation of regulatory strategies for the sex determination factor transformer-2.Molecular and cellular biology · 1997Article
- Muscle-specific splicing enhancers regulate inclusion of the cardiac troponin T alternative exon in embryonic skeletal muscle.Molecular and cellular biology · 1996Article
- Selection of novel exon recognition elements from a pool of random sequences.Molecular and cellular biology · 1995Article
- Relative exon affinities and suboptimal splice site signals lead to non-equivalence of two cassette exons.Nucleic acids research · 1995Article
- A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer.Molecular and cellular biology · 1995Article
- The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.The EMBO journal · 1995Article
- Cooperation of 5' and 3' processing sites as well as intron and exon sequences in calcitonin exon recognition.Nucleic acids research · 1995Article
- Identification and characterization by antisense oligonucleotides of exon and intron sequences required for splicing.Molecular and cellular biology · 1994Article
- SR proteins promote the first specific recognition of Pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex.Molecular and cellular biology · 1994Article
- Nonsense but not missense mutations can decrease the abundance of nuclear mRNA for the mouse major urinary protein, while both types of mutations can facilitate exon skipping.Molecular and cellular biology · 1994Article
- Alternative splicing of beta-tropomyosin pre-mRNA: multiple cis-elements can contribute to the use of the 5'- and 3'-splice sites of the nonmuscle/smooth muscle exon 6.Nucleic acids research · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cardiac troponin T (cTNT) pre-mRNA contains a single alternative exon (exon 5) which is either included or excluded from the processed mRNA. Using transient transfection of cTNT minigenes, we have previously localized pre-mRNA cis elements required for exon 5 alternative splicing to three small regions of the pre-mRNA which include exons 4, 5, and 6. In the present study, nucleotide substitutions were introduced into the region containing exon 5 to begin to define specific nucleotides required for exon 5 alternative splicing. A mutation within the 5' splice site flanking the cTNT alternative exon that increases its homology to the consensus sequence improves splicing efficiency and leads to increased levels of mRNAs that include the alternative exon. Surprisingly, substitution of as few as four nucleotides within the alternative exon disrupts cTNT pre-mRNA alternative splicing and prevents recognition of exon 5 as a bona fide exon. These results establish that the cTNT alternative exon contains information in cis that is required for its recognition by the splicing machinery.
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