Evidence map›Paper›PMID 27981242›Full record

ArticleBioengineering & translational medicine2016

Reducing neuroinflammation by delivery of IL-10 encoding lentivirus from multiple-channel bridges.

Daniel J Margul, Jonghyuck Park, Ryan M Boehler, Dominique R Smith, Mitchell A Johnson, Dylan A McCreedy, Ting He, Aishani Ataliwala, Todor V Kukushliev, Jesse Liang and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineering & translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Clickable Biomaterials for Modulating Neuroinflammation.International journal of molecular sciences · 2022
    Review
  7. Immunomodulatory Strategies for Spinal Cord Injury.Biomedical journal of scientific & technical research · 2022
    Article
  8. Review
  9. Review
  10. Article
  11. Lentiviral Interleukin-10 Gene Therapy Preserves Fine Motor Circuitry and Function After a Cervical Spinal Cord Injury in Male and Female Mice.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2021
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Regenerative Therapies for Spinal Cord Injury.Tissue engineering. Part B, Reviews · 2019
    Review
  18. A single combination gene therapy treats multiple age-related diseases.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 1 country.

Daniel J MargulDept. of Biomedical Engineering Northwestern University Evanston IL, 48109; Dept. of Biomedical Engineering University of Michigan Ann Arbor MI, 48109.
Jonghyuck ParkDept. of Biomedical Engineering University of Michigan Ann Arbor MI, 48109.
Ryan M BoehlerDept. of Chemical and Biological Engineering Northwestern University Evanston IL, 48109.
Dominique R SmithDept. of Biomedical Engineering Northwestern University Evanston IL, 48109; Dept. of Biomedical Engineering University of Michigan Ann Arbor MI, 48109.
Mitchell A JohnsonDept. of Biomedical Engineering University of Michigan Ann Arbor MI, 48109.
Dylan A McCreedyDept. of Biomedical Engineering University of Michigan Ann Arbor MI, 48109; Dept. of Chemical and Biological Engineering Northwestern University Evanston IL, 48109.
Ting HeDept. of Chemical and Biological Engineering Northwestern University Evanston IL, 48109.
Aishani AtaliwalaDept. of Bioengineering University of California Los Angeles Los Angeles CA, 90095.
Todor V KukushlievDept. of Chemical and Biological Engineering Northwestern University Evanston IL, 48109.
Jesse LiangDept. of Bioengineering University of California Los Angeles Los Angeles CA, 90095.
Alireza SohrabiDept. of Bioengineering University of California Los Angeles Los Angeles CA, 90095.
Ashley G GoodmanDept. of Chemical and Biological Engineering Northwestern University Evanston IL, 48109.
Christopher M WalthersDept. of Bioengineering University of California Los Angeles Los Angeles CA, 90095.
Lonnie D SheaDept. of Biomedical Engineering University of Michigan Ann Arbor MI, 48109; Dept. of Chemical Engineering University of Michigan Ann Arbor MI, 48109.
Stephanie K SeidlitsDept. of Chemical and Biological Engineering Northwestern University EvanstonIL, 48109; Dept. of Bioengineering University of California Los Angeles Los Angeles CA, 90095; Brain Research Institute University of California Los Angeles Los Angeles CA, 90095; Jonsson Comprehensive Cancer Center University of California Los Angeles Los Angeles CA, 90024.
University of Michigan–Ann Arbor · USNorthwestern University · USUniversity of California, Los Angeles · USUSC Norris Comprehensive Cancer Center · US

Funding

Controlled Release Scaffolds for Nerve RegenerationR01EB005678 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ANDERSON, AILEEN J, SHEA, LONNIE D · 2007 to 2018
$6.0M
An in vivo metastasis sensorR01CA173745 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BACKMAN, VADIM, SHEA, LONNIE D · 2012 to 2016
$4.2M
NCI NIH HHS R01 CA173745NIBIB NIH HHS R01 EB005678
6 · The paper itself

Abstract

The spinal cord is unable to regenerate after injury largely due to growth-inhibition by an inflammatory response to the injury that fails to resolve, resulting in secondary damage and cell death. An approach that prevents inhibition by attenuating the inflammatory response and promoting its resolution through the transition of macrophages to anti-inflammatory phenotypes is essential for the creation of a growth permissive microenvironment. Viral gene delivery to induce the expression of anti-inflammatory factors provides the potential to provide localized delivery to alter the host inflammatory response. Initially, we investigated the effect of the biomaterial and viral components of the delivery system to influence the extent of cell infiltration and the phenotype of these cells. Bridge implantation reduces antigen-presenting cell infiltration at day 7, and lentivirus addition to the bridge induces a transient increase in neutrophils in the spinal cord at day 7 and macrophages at day 14. Delivery of a lentivirus encoding IL-10, an anti-inflammatory factor that inhibits immune cell activation and polarizes the macrophage population towards anti-inflammatory phenotypes, reduced neutrophil infiltration at both day 7 and day 28. Though IL-10 lentivirus did not affect macrophages number, it skewed the macrophage population toward an anti-inflammatory M2 phenotype and altered macrophage morphology. Additionally, IL-10 delivery resulted in improved motor function, suggesting reduced secondary damage and increased sparing. Taken together, these results indicate that localized expression of anti-inflammatory factors, such as IL-10, can modulate the inflammatory response following spinal cord injury, and may be a key component of a combinatorial approach that targets the multiple barriers to regeneration and functional recovery.

Indexed as

gene deliveryimmune modulationmultiple‐channel bridgespinal cord injury

Identifiers

PMID27981242
PMCPMC5125399
OpenAlexW2552399398

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.