ArticleBiotechnology for biofuels2016
Systematic engineering of pentose phosphate pathway improves
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 55 citations in OpenAlex.
- Enhancement and balance of 1,4-butanediamine biosynthesis in Escherichia coli based on modular metabolic engineering.Journal, genetic engineering & biotechnology · 2026Article
- Product-Responsive Regulation of NADPH and Malonyl-CoA Allocation Enhances 3-Hydroxypropionic Acid Production inACS synthetic biology · 2026Article
- Efficient synthesis of l-DOPA inSynthetic and systems biotechnology · 2026Article
- Redox engineering of thermophilic fungusMycology · 2026Article
- Adapting OptCouple to Identify Strategies with Increased Product Yields in Community Cohorts ofMetabolites · 2025Article
- Rewiring metabolic flux to simultaneously improve malate production and eliminate by-product succinate accumulation by Myceliophthora thermophila.Microbial biotechnology · 2024Article
- Streamlined identification of strain engineering targets for bioprocess improvement using metabolic pathway enrichment analysis.Scientific reports · 2023Article
- Characterization of the enzyme kinetics of EMP and HMP pathway inFrontiers in bioengineering and biotechnology · 2023Article
- Intertwined Relationship of Mitochondrial Metabolism, Gut Microbiome and Exercise Potential.International journal of molecular sciences · 2022Review
- Enhancement of β-Alanine Biosynthesis inBiology · 2021Article
- Application of a dissolved oxygen control strategy to increase the expression of Streptococcus suis glutamate dehydrogenase in Escherichia coli.World journal of microbiology & biotechnology · 2021Article
- Effective production of succinic acid from coconut water (Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- Engineering ofBiotechnology for biofuels · 2018Article
- Overexpression of a CMicrobial cell factories · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSuccinate biosynthesis of
resultsAltering expression levels of different PPP enzymes have distinct effects on succinate production. Specifically, increased expression of five enzymes, i.e., Zwf, Pgl, Gnd, Tkt, and Tal, contributes to increased succinate production, while the increased expression of two enzymes, i.e., Rpe and Rpi, significantly decreases succinate production. Modular engineering strategy is employed to decompose PPP into three modules according to position and function. Engineering of Zwf/Pgl/Gnd and Tkt/Tal modules effectively increases succinate yield and production, while engineering of Rpe/Rpi module decreases. Imbalance of enzymatic reactions in PPP is alleviated using MMME approach. Finally, combinational utilization of engineered PPP and SthA transhydrogenase enables succinate yield up to 1.61 mol/mol glucose, which is 94% of theoretical maximum yield (1.71 mol/mol) and also the highest succinate yield in minimal medium to our knowledge.
conclusionsIn summary, we systematically engineered the PPP for improving the supply of reducing equivalents and thus succinate production. Besides succinate, these PPP engineering strategies and conclusions can also be applicable to the production of other reducing equivalent-dependent biorenewables.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.