ArticleMetabolomics : Official journal of the Metabolomic Society2017
Stability of targeted metabolite profiles of urine samples under different storage conditions.
Article in Metabolomics : Official journal of the Metabolomic Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- Systematic Review: Urine Biomarker Discovery for Inflammatory Bowel Disease Diagnosis.International journal of molecular sciences · 2023Pooled it
- Retrospective evaluation of lipiduria in urine from 140 dogs and 74 cats.Journal of veterinary internal medicine · 2026Article
- Detection of Mycobacterium tuberculosis antigens in urinary extracellular vesicles using real-time immuno-PCR: A non-invasive diagnostic approach for pulmonary tuberculosis.Scientific reports · 2026Article
- Cortisol Stability at 16 and 30 Years in Urine Specimens Stored at -20°C.American journal of human biology : the official journal of the Human Biology Council · 2026Article
- Stability of Nε-Carboxymethyllysine and Nε-Carboxyethyllysine in Canine Urine Under Extended Room Temperature Storage.Animals : an open access journal from MDPI · 2026Article
- Early prediction of bronchopulmonary dysplasia by urinary metabolomics: a case-control study.Thorax · 2026Observational
- Neuronal and Glial Biomarkers in Urine of Athletes with Different Risks of Head Trauma to Monitor Sports-Related Concussions.Molecular neurobiology · 2025Article
- Influence of pre-analytical factors and biological variability on the urinary volatilome.Analytical and bioanalytical chemistry · 2025Article
- Prediction of irritable bowel syndrome by integrating urine metabolites and gut microbiota.Scientific reports · 2025Article
- Associations of chronic liver disease and liver cancer with glyphosate and its metabolites in Thailand.International journal of cancer · 2025Article
- Urinary Metabolomic Profile is Minimally Impacted by Common Storage Conditions and Additives.International urogynecology journal · 2025Article
- BD Vacutainer™ Urine Culture & Sensitivity Preservative PLUS Plastic Tubes Minimize the Harmful Impact of Stressors Dependent on Temperature and Time Storage in Uropathogenic Bacteria.Journal of clinical medicine · 2024Article
- Impact of the COVID-19 Pandemic on Obesity, Metabolic Parameters and Clinical Values in the South Korean Adult Population.Journal of clinical medicine · 2024Article
- Stability of volatile organic compound metabolites in urine at various storage temperatures and freeze-thaw cycles for 8 months.Environmental pollution (Barking, Essex : 1987) · 2024Article
- Effect of very long-term storage and multiple freeze and thaw cycles on 11-dehydro-thromboxane-BScientific reports · 2024Article
- Microbiome metabolite quantification methods enabling insights into human health and disease.Methods (San Diego, Calif.) · 2024Review
- Effects of Preanalytical Sample Collection and Handling on Comprehensive Metabolite Measurements in Human Urine Biospecimens.medRxiv : the preprint server for health sciences · 2024Article
- Investigation of select radionuclides stability in urine under various conditions for liquid scintillation counting (LSC).Journal of radioanalytical and nuclear chemistry · 2024Article
- The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies.Journal of proteome research · 2022Article
- From bedside to bench-practical considerations to avoid pre-analytical pitfalls and assess sample quality for high-resolution metabolomics and lipidomics analyses of body fluids.Analytical and bioanalytical chemistry · 2021Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionFew studies have investigated the influence of storage conditions on urine samples and none of them used targeted mass spectrometry (MS).
objectivesWe investigated the stability of metabolite profiles in urine samples under different storage conditions using targeted metabolomics.
methodsPooled, fasting urine samples were collected and stored at -80 °C (biobank standard), -20 °C (freezer), 4 °C (fridge), ~9 °C (cool pack), and ~20 °C (room temperature) for 0, 2, 8 and 24 h. Metabolite concentrations were quantified with MS using the AbsoluteIDQ™ p150 assay. We used the Welch-Satterthwaite-test to compare the concentrations of each metabolite. Mixed effects linear regression was used to assess the influence of the interaction of storage time and temperature.
resultsThe concentrations of 63 investigated metabolites were stable at -20 and 4 °C for up to 24 h when compared to samples immediately stored at -80 °C. When stored at ~9 °C for 24 h, few amino acids (Arg, Val and Leu/Ile) significantly decreased by 40% in concentration (
conclusionOur findings indicate that 78% of quantified metabolites were stable for all examined storage conditions. Particularly, some amino acid concentrations were sensitive to changes after prolonged storage at room temperature. Shipping or storing urine samples on cool packs or at room temperature for more than 8 h and multiple numbers of freeze and thaw cycles should be avoided.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.