ArticleBMC veterinary research2016
A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences.
Article in BMC veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Evaluation of two inactivated vaccines against Campylobacter fetus in an experimental bovine model: immunological survey and genital clearance in heifers.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Quadruplex qPCR for detection and discrimination of C. Coli,C. fetus, and C. Jejuni from other Campylobacter species in chicken and sheep meat.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases.Veterinary research communications · 2024Article
- Bovine campylobacteriosis in bulls: insights in the conventional and molecular diagnosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion.Pathogens (Basel, Switzerland) · 2023Article
- Comparative genomic analysis ofFrontiers in microbiology · 2023Article
- Diagnostic Investigation of 100 Cases of Abortion in Sheep in Uruguay: 2015-2021.Frontiers in veterinary science · 2022Article
- Molecular diagnosis of bovine genital campylobacteriosis using high-resolution melting analysis.Frontiers in microbiology · 2022Article
- Accurate and fast identification ofVeterinary and animal science · 2021Article
- Phylogenomic Analysis ofFrontiers in microbiology · 2020Article
- Review
- Diagnosis of Bovine Genital Campylobacteriosis in South America.Frontiers in veterinary science · 2018Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCampylobacter fetus is a pathogen of major concern for animal and human health. The species shows a great intraspecific variation, with three subspecies: C. fetus subsp. fetus, C. fetus subsp. venerealis, and C. fetus subsp. testudinum. Campylobacter fetus fetus affects a broad range of hosts and induces abortion in sheep and cows. Campylobacter fetus venerealis is restricted to cattle and causes the endemic disease bovine genital campylobacteriosis, which triggers reproductive problems and is responsible for major economic losses. Campylobacter fetus testudinum has been proposed recently based on genetically divergent strains isolated from reptiles and humans. Both C. fetus fetus and C. fetus testudinum are opportunistic pathogens for immune-compromised humans. Biochemical tests remain as the gold standard for identifying C. fetus but the fastidious growing requirements and the lack of reliability and reproducibility of some biochemical tests motivated the development of molecular diagnostic tools. These methods have been successfully tested on bovine isolates but fail to detect some genetically divergent strains isolated from other hosts. The aim of the present study was to develop a highly specific molecular assay to identify and quantify C. fetus strains.
resultsWe developed a highly sensitive real-time PCR assay that targets a unique region of the 16S rRNA gene. This assay successfully detected all C. fetus strains, including those that were negative for the cstA gene-based assay used as a standard for molecular C. fetus identification. The assay showed high specificity and absence of cross-reactivity with other bacterial species. The analytical testing of the assay was determined using a standard curve. The assay demonstrated a wide dynamic range between 10
conclusionsThe possibility to characterize samples in a rapid, sensitive and reproducible way makes this assay a good option to establish a new standard in molecular identification and quantification of C. fetus species.
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