ArticlePloS one2016
Massively Parallelized Pollen Tube Guidance and Mechanical Measurements on a Lab-on-a-Chip Platform.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- An Automated Lab-On-A-Chip Approach for Pollen Tube Growth Manipulation in a Controlled Chemical Environment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Applications and Future Prospects of Micro/Nanorobots Utilizing Diverse Biological Carriers.Micromachines · 2023Review
- Cultivation and Imaging ofBioengineering (Basel, Switzerland) · 2022Article
- Microfluidics-Based Bioassays and Imaging of Plant Cells.Plant & cell physiology · 2021Review
- 3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy.Nature communications · 2021Article
- Iterative subtraction facilitates automated, quantitative analysis of multiple pollen tube growth features.Plant reproduction · 2019Article
- The brown algal mode of tip growth: Keeping stress under control.PLoS biology · 2019Article
- Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces.Journal of visualized experiments : JoVE · 2018Article
- Single measurement detection of individual cell ionic oscillations using an n-type semiconductor - electrolyte interface.Scientific reports · 2018Article
- Cell-cell communications and molecular mechanisms in plant sexual reproduction.Journal of plant research · 2018Review
- Capability of tip-growing plant cells to penetrate into extremely narrow gaps.Scientific reports · 2017Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen tubes are used as a model in the study of plant morphogenesis, cellular differentiation, cell wall biochemistry, biomechanics, and intra- and intercellular signaling. For a "systems-understanding" of the bio-chemo-mechanics of tip-polarized growth in pollen tubes, the need for a versatile, experimental assay platform for quantitative data collection and analysis is critical. We introduce a Lab-on-a-Chip (LoC) concept for high-throughput pollen germination and pollen tube guidance for parallelized optical and mechanical measurements. The LoC localizes a large number of growing pollen tubes on a single plane of focus with unidirectional tip-growth, enabling high-resolution quantitative microscopy. This species-independent LoC platform can be integrated with micro-/nano-indentation systems, such as the cellular force microscope (CFM) or the atomic force microscope (AFM), allowing for rapid measurements of cell wall stiffness of growing tubes. As a demonstrative example, we show the growth and directional guidance of hundreds of lily (Lilium longiflorum) and Arabidopsis (Arabidopsis thaliana) pollen tubes on a single LoC microscopy slide. Combining the LoC with the CFM, we characterized the cell wall stiffness of lily pollen tubes. Using the stiffness statistics and finite-element-method (FEM)-based approaches, we computed an effective range of the linear elastic moduli of the cell wall spanning the variability space of physiological parameters including internal turgor, cell wall thickness, and tube diameter. We propose the LoC device as a versatile and high-throughput phenomics platform for plant reproductive and development biology using the pollen tube as a model.
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Registered trials
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