Evidence map›Paper›PMID 27975320›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization.

Yanli Qin, Yong-Xiang Wang, Jiming Zhang, Jisu Li, Shuping Tong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 70% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Yanli QinLiver Research Center, Rhode Island Hospital, Brown University, Providence, RI, USA.
Yong-Xiang WangDepartment of Pathogen Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Jiming ZhangDepartment of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
Jisu LiLiver Research Center, Rhode Island Hospital, Brown University, Providence, RI, USA.
Shuping TongLiver Research Center, Rhode Island Hospital, Brown University, Providence, RI, USA. shuping_tong_md@brown.edu.
Fudan University · CNBrown University · USHuashan Hospital · CNRhode Island Hospital · US

Funding

Hepatitis B virus vaccine escape mutantsR01AI116639 · NIAID · RHODE ISLAND HOSPITAL · PI TONG, SHUPING · 2015 to 2019
$1.8M
Hepatitis B virus genotypes B and CR21AI103648 · NIAID · RHODE ISLAND HOSPITAL · PI TONG, SHUPING · 2013 to 2014
$437k
Control of HBV replication at the step of core and P protein translationR21AI107618 · NIAID · RHODE ISLAND HOSPITAL · PI LI, JISU · 2013 to 2014
$423k
NIAID NIH HHS R01 AI116639NIAID NIH HHS R21 AI103648NIAID NIH HHS R21 AI107618
6 · The paper itself

Abstract

Hepatitis B virus (HBV) infection can be associated with a spectrum of clinical outcomes. Transient transfection of the clinical HBV isolates in human hepatoma cell lines can establish their biological properties to shed light on their different pathogenic potentials, yet very few clinical HBV isolates have been functionally characterized so far. The technical challenges include faithful amplification of the full-length HBV genome from clinical samples and conversion into a replication-competent form. We have improved a published method to amplify the full-length HBV genome from blood samples. Two alternative approaches are used to render the cloned HBV genome replication competent: release and circularization of the 3.2-kb HBV genome prior to each transfection experiment or conversion of the monomeric clone into a tandem dimer version.

Indexed as

Genome, ViralCell LineCloning, MolecularDNA, CircularDNA, ViralHepatitis BHepatitis B virusHumansPolymerase Chain ReactionRNA, ViralTranscription, GeneticTransfectionVirionVirus ReplicationDNA, CircularDNA, ViralRNA, ViralHBV clinical isolatesTandem dimerTransfection

Identifiers

PMID27975320
PMCPMC6348386
OpenAlexW2566467394

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.