ArticleParasitology research2017
Nested PCR targeting intergenic spacer (IGS) in genotyping of Giardia duodenalis isolated from symptomatic and asymptomatic infected Egyptian school children.
Article in Parasitology research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Global epidemiology of Giardia duodenalis infection in cancer patients: a systematic review and meta-analysis.International health · 2022Pooled it
- Genotyping and distribution ofParasitology · 2026Article
- Molecular characterization and risk analysis of Giardia duodenalis assemblages in corticosteroid-treated and non-treated patients in Ismailia, Arab Republic of Egypt.Gut pathogens · 2024Article
- Molecular characterization and risk analysis of Giardia duodenalis assemblages in corticosteroid-treated and non-treated patients in Ismailia, Arab Republic of Egypt.Gut pathogens · 2024Article
- Rapid visual detection of Giardia duodenalis in faecal samples using an RPA-CRISPR/Cas12a system.Parasitology research · 2024Article
- Prevalence ofVeterinary world · 2024Article
- Microscopy detection and molecular characterisation ofFrontiers in public health · 2024Article
- The prevalence of potentially zoonotic intestinal parasites in dogs and cats in Moscow, Russia.Helminthologia · 2023Article
- Detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Symptomatic and Asymptomatic Protist Infections in Hospital Inpatients in Southwestern China.Pathogens (Basel, Switzerland) · 2021Article
- The controversies surroundingCurrent research in parasitology & vector-borne diseases · 2021Review
- Giardia duodenalis Virulence - "To Be, or Not To Be".Current tropical medicine reports · 2021Review
- Prevalence and genotypic characterization ofFood and waterborne parasitology · 2020Article
- Prevalence and Zoonotic Potential ofAnimals : an open access journal from MDPI · 2019Article
- A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt.Pathogens (Basel, Switzerland) · 2017Review
- Molecular detection and genotyping ofTropical parasitologyArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Distinct sequences of Giardia duodenalis assemblages raised the hypothesis that certain assemblages may contribute to its clinical outcome. However, sequences analysis is time consuming, expensive, and needs many manual operations. Nested PCR targeting intergenic spacer (IGS) region was applied successfully to genotype G. duodenalis. This study aimed to identify the prevalence of G. duodenalis assemblages among giardiasis school children and its relation to the presence of symptoms using nested IGS/PCR. Of 65 microscopically confirmed Giardia-positive samples, 65 samples were genotyped proving high sensitivity (92.3%) of IGS/PCR. Negative IGS/PCR samples were also negative for β-giardin gene. Subassemblage AI was the commonest with 66.6% (20/30) among asymptomatic children compared to 53.3% (16/30) of symptomatic, while assemblage B was found in 40% (12/30) of symptomatic compared to 20% (6/30) of asymptomatic. The difference was significant. AII was only found in asymptomatic with 13.4% (4/30), while mixed infections (AI&B) were recorded only in 6.6% (2/30) of symptomatic group. A significant relation was found between younger children susceptibility for AI and B infections as presented in 77.7 (12/16) and 83.3% (10/12) of symptomatic, respectively, and 80 (16/80) and 33.4% (2/4) of asymptomatic, respectively. Significant relations were found between AI with intermittent diarrhea and B with chronic. A significant relation was found between assemblage distributions and heavy infection intensity. In conclusion, higher incidence of assemblage B among symptomatic children compared to asymptomatic could denote its possible pathogenic potential.
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