ArticleJournal of lipid research2017
An optimized method for measuring fatty acids and cholesterol in stable isotope-labeled cells.
Article in Journal of lipid research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A protocol for noninvasive quantification of dietary fat absorption in mice.STAR protocols · 2026Article
- Physiological Differences in Sebum Composition in Regularly Menstruating Healthy Women.The Journal of dermatology · 2025Article
- The designer cytokine IC7Fc attenuates atherosclerosis development by targeting hyperlipidemia in mice.Science advances · 2025Article
- Defining STING-sterol interactions with chemoproteomics.RSC chemical biology · 2025Article
- Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide.Journal of visualized experiments : JoVE · 2023Article
- Automated Trimethyl Sulfonium Hydroxide Derivatization Method for High-Throughput Fatty Acid Profiling by Gas Chromatography-Mass Spectrometry.Molecules (Basel, Switzerland) · 2021Article
- Production and Application of Stable Isotope-Labeled Internal Standards for RNA Modification Analysis.Genes · 2019Article
- Development and Application of FASA, a Model for Quantifying Fatty Acid Metabolism Using Stable Isotope Labeling.Cell reports · 2018Article
- Stable Isotope Labeling Highlights Enhanced Fatty Acid and Lipid Metabolism in Human Acute Myeloid Leukemia.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Stable isotope labeling has become an important methodology for determining lipid metabolic parameters of normal and neoplastic cells. Conventional methods for fatty acid and cholesterol analysis have one or more issues that limit their utility for in vitro stable isotope-labeling studies. To address this, we developed a method optimized for measuring both fatty acids and cholesterol from small numbers of stable isotope-labeled cultured cells. We demonstrate quantitative derivatization and extraction of fatty acids from a wide range of lipid classes using this approach. Importantly, cholesterol is also recovered, albeit at a modestly lower yield, affording the opportunity to quantitate both cholesterol and fatty acids from the same sample. Although we find that background contamination can interfere with quantitation of certain fatty acids in low amounts of starting material, our data indicate that this optimized method can be used to accurately measure mass isotopomer distributions for cholesterol and many fatty acids isolated from small numbers of cultured cells. Application of this method will facilitate acquisition of lipid parameters required for quantifying flux and provide a better understanding of how lipid metabolism influences cellular function.
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Registered trials
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