ArticleThe Journal of eukaryotic microbiology2017
Haptophyte Diversity and Vertical Distribution Explored by 18S and 28S Ribosomal RNA Gene Metabarcoding and Scanning Electron Microscopy.
Article in The Journal of eukaryotic microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment.Molecular ecology · 2022Pooled it
- Spatial Distributions of Active Pico- and Nano-Haptophytes (Eukaryota, Hacrobia) in the Tropical and Subtropical Western Pacific Ocean.Microorganisms · 2026Article
- Picophytoplankton implicated in productivity and biogeochemistry in the North Pacific Transition Zone.mSystems · 2026Article
- Article
- Exploring the Regional Diversity of Eukaryotic Phytoplankton in the English Channel by Combining High-Throughput Approaches.MicrobiologyOpen · 2025Article
- A protozoan perspective on climate change and biosafety threats: differences in testate amoebae in lakes in forest-swamp and forest-steppe zones in Western Siberia.Applied and environmental microbiology · 2025Article
- The protist community of the oligotrophic waters of the Gulf of Mexico is distinctly shaped by depth-specific physicochemical conditions during the warm season.FEMS microbiology ecology · 2025Article
- Article
- Group 2i Isochrysidales produce characteristic alkenones reflecting sea ice distribution.Nature communications · 2021Article
- Distribution of Chromophytic Phytoplankton in the Eddy-Induced Upwelling Region of the West Pacific Ocean Revealed UsingFrontiers in microbiology · 2021Article
- Phylogenetic diversity in freshwater-dwelling Isochrysidales haptophytes with implications for alkenone production.Geobiology · 2019Article
- Protist Diversity and Seasonal Dynamics in Skagerrak Plankton Communities as Revealed by Metabarcoding and Microscopy.The Journal of eukaryotic microbiology · 2019Article
- Scalable methods for analyzing and visualizing phylogenetic placement of metagenomic samples.PloS one · 2019Article
- Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haptophyta encompasses more than 300 species of mostly marine pico- and nanoplanktonic flagellates. Our aims were to investigate the Oslofjorden haptophyte diversity and vertical distribution by metabarcoding, and to improve the approach to study haptophyte community composition, richness and proportional abundance by comparing two rRNA markers and scanning electron microscopy (SEM). Samples were collected in August 2013 at the Outer Oslofjorden, Norway. Total RNA/cDNA was amplified by haptophyte-specific primers targeting the V4 region of the 18S, and the D1-D2 region of the 28S rRNA. Taxonomy was assigned using curated haptophyte reference databases and phylogenetic analyses. Both marker genes showed Chrysochromulinaceae and Prymnesiaceae to be the families with highest number of Operational Taxonomic Units (OTUs), as well as proportional abundance. The 18S rRNA data set also contained OTUs assigned to eight supported and defined clades consisting of environmental sequences only, possibly representing novel lineages from family to class. We also recorded new species for the area. Comparing coccolithophores by SEM with metabarcoding shows a good correspondence with the 18S rRNA gene proportional abundances. Our results contribute to link morphological and molecular data and 28S to 18S rRNA gene sequences of haptophytes without cultured representatives, and to improve metabarcoding methodology.
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