ArticleJournal of musculoskeletal & neuronal interactions2016
Alterations in gene expression precede sarcopenia and osteopenia in botulinum toxin immobilized mice.
Article in Journal of musculoskeletal & neuronal interactions, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Article
- Teriparatide and Abaloparatide Have a Similar Effect on Bone in Mice.Frontiers in endocrinology · 2021Article
- Article
- Zoledronic acid prevents disuse osteopenia and augments gene expression of osteoclastic differentiation markers in mice.Journal of musculoskeletal & neuronal interactions · 2018Article
- Disuse osteopenia induced by botulinum toxin is similar in skeletally mature young and aged female C57BL/6J mice.Journal of bone and mineral metabolism · 2018Article
- Pantoprazole, a proton pump inhibitor, does not prevent botulinum toxin induced disuse osteopenia in mice.Journal of musculoskeletal & neuronal interactions · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo investigate alteration of bone and muscle gene expression at different time points during 3 weeks of botulinum toxin (BTX) induced immobilization and how this correlate with conventional analysis of bone and muscle.
methodsThirty-five 16-week-old female C57BL/6-mice were investigated; 15 were injected with BTX, 15 served as age-matched controls, and 5 as baseline. 5 BTX-injected and 5 control mice were euthanized after 1, 2, and 3 weeks. Analysis included RT-qPCR, dynamic bone histomorphometry, DEXA, µCT, mechanical testing, and muscle cell cross-sectional-area (CSA).
resultsGenes related to osteoblasts were expressed at a lower level after 1 week, but not after 2 and 3 weeks of disuse. Moreover, genes related to osteoclasts were expressed at a higher level after 1 and 2 weeks of disuse, whereafter they approached the level of the controls. Genes related to muscle atrophy were upregulated 1 and 2 weeks after the BTX-injection, but not after 3 weeks. In contrast, deterioration of bone microstructure and strength, and reduction in muscle cell CSA were most evident after 3 weeks of disuse.
conclusionsGene expression should be investigated during the first two weeks of immobilization, whereas changes in bone microstructure and muscle cell CSA are most prominent after 3 weeks of immobilization.
Indexed as
Identifiers
27973388PMC5259577W2562662540What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.