ArticleScientific reports2016
Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 23 citations in OpenAlex.
- Early detection and tracking of wood borers using improved environmental DNA aggregation and TaqMan quantitative PCR approaches in forests.Insect science · 2026Article
- Unlocking rivers' hidden diversity and ecological status using DNA metabarcoding in Northwest Spain.Ecology and evolution · 2024Article
- Environmental (e)RNA advances the reliability of eDNA by predicting its age.Scientific reports · 2021Article
- Invasion genetics from eDNA and thousands of larvae: A targeted metabarcoding assay that distinguishes species and population variation of zebra and quagga mussels.Ecology and evolution · 2019Article
- Review
- Conventional versus real-time quantitative PCR for rare species detection.Ecology and evolution · 2018Article
- Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.PloS one · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations.
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Registered trials
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