Evidence map›Paper›PMID 27965062›Full record

ArticleAnalytical biochemistry2017

Gel filtration of dilute human embryonic hemoglobins reveals basis for their increased oxygen binding.

Lois R Manning, Anthony M Popowicz, Julio C Padovan, Brian T Chait, James M Manning

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Lois R ManningDepartment of Biology, Northeastern University, Boston, MA 02115, USA.
Anthony M PopowiczInformation Technology, The Rockefeller University, New York, NY 10065, USA.
Julio C PadovanLaboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY 10065, USA.
Brian T ChaitLaboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY 10065, USA.
James M ManningDepartment of Biology, Northeastern University, Boston, MA 02115, USA. Electronic address: j.manning@neu.edu.
Rockefeller University · USNortheastern University · US

Funding

YNG1 PHD BINDING TO H3 3METHYL AT K4 PROMOTES TRANSCRIPTION AT TARGETED ORFSP41RR000862 · NCRR · ROCKEFELLER UNIVERSITY · PI DARNELL, JAMES E · 1985 to 2011
$16.1M
National Resource for Mass Spectrom of Biological MacromoleculesP41GM103314 · NIGMS · ROCKEFELLER UNIVERSITY · PI CHAIT, BRIAN T · 2012 to 2017
$9.9M
NCRR NIH HHS P41 RR000862NIGMS NIH HHS P41 GM103314
6 · The paper itself

Abstract

This report establishes a correlation between two known properties of the human embryonic hemoglobins-- their weak subunit assemblies as demonstrated here by gel filtration at very dilute protein concentrations and their high oxygen affinities and reduced cooperativities reported previously by others but without a mechanistic basis. We demonstrate here that their high oxygen affinities are a consequence of their weak assemblies. Weak vs strong hemoglobin tetramers represent a regulatory mechanism to modulate oxygen binding capacity by altering the equilibrium between the various steps in the assembly process that can be described as an inverse allosteric effect.

Indexed as

Allosteric RegulationChromatography, GelEmbryo, MammalianHemoglobinsHemoglobins, AbnormalHumansHydrogen-Ion ConcentrationKineticsOxygenProtein BindingThermodynamicsHemoglobinsHemoglobins, AbnormalOxygenDevelopmentGel filtrationHemoglobinOxygen affinitySubunit assembly

Identifiers

PMID27965062
PMCPMC5237603
OpenAlexW2560126069

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.