ArticlePloS one2016
Comprehensive Virus Detection Using Next Generation Sequencing in Grapevine Vascular Tissues of Plants Obtained from the Wine Regions of Bohemia and Moravia (Czech Republic).
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Unleashing the potential of high-throughput sequencing for plant virus and viroid detection in Mexico.Frontiers in microbiology · 2025Article
- Multiple Infections with Viruses of the FamilyViruses · 2024Article
- Application of High-Throughput Sequencing for Comprehensive Virome Profiling in Grapevines Shows Yellows in Iran.Viruses · 2024Article
- The First Virome of a Russian Vineyard.Plants (Basel, Switzerland) · 2023Article
- The genetic variability of grapevine Pinot gris virus (GPGV) in Australia.Virology journal · 2023Article
- Article
- Detection and discovery of plant viruses in soybean by metagenomic sequencing.Virology journal · 2022Article
- Article
- Virome Status of Preclonal Candidates of Grapevine Varieties (Frontiers in microbiology · 2022Article
- High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia.Viruses · 2021Article
- Article
- Survey and Diversity ofGenes · 2020Article
- MicroRNAs inGenes · 2019Article
- List of non-EU viruses and viroids ofEFSA journal. European Food Safety Authority · 2019Article
- High-Throughput Sequencing Analysis of the Bacterial Community in Stone Fruit Phloem Tissues Infected by "Candidatus Phytoplasma prunorum".Microbial ecology · 2019Article
- Peach RNA viromes in six different peach cultivars.Scientific reports · 2018Article
- Comparison of Serological and Molecular Methods With High-Throughput Sequencing for the Detection and Quantification of Grapevine Fanleaf Virus in Vineyard Samples.Frontiers in microbiology · 2018Article
- Small RNA-Omics for Plant Virus Identification, Virome Reconstruction, and Antiviral Defense Characterization.Frontiers in microbiology · 2018Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comprehensive next generation sequencing virus detection was used to detect the whole spectrum of viruses and viroids in selected grapevines from the Czech Republic. The novel NGS approach was based on sequencing libraries of small RNA isolated from grapevine vascular tissues. Eight previously partially-characterized grapevines of diverse varieties were selected and subjected to analysis: Chardonnay, Laurot, Guzal Kara, and rootstock Kober 125AA from the Moravia wine-producing region; plus Müller-Thurgau and Pinot Noir from the Bohemia wine-producing region, both in the Czech Republic. Using next generation sequencing of small RNA, the presence of 8 viruses and 2 viroids were detected in a set of eight grapevines; therefore, confirming the high effectiveness of the technique in plant virology and producing results supporting previous data on multiple infected grapevines in Czech vineyards. Among the pathogens detected, the Grapevine rupestris vein feathering virus and Grapevine yellow speckle viroid 1 were recorded in the Czech Republic for the first time.
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