Evidence map›Paper›PMID 27959493›Full record

ArticleAnalytical chemistry2017

Droplet Microfluidic Flow Cytometer For Sorting On Transient Cellular Responses Of Genetically-Encoded Sensors.

Brett L Fiedler, Steven Van Buskirk, Kyle P Carter, Yan Qin, Margaret C Carpenter, Amy E Palmer, Ralph Jimenez

Abstract read
In one paragraph

Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. ZIP3 is essential for the encephalitogenic function of pTh17 cells by regulating intracellular zinc levels.Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) · 2026
    Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Human cells experience a ZnCell reports · 2023
    Article
  14. Review
  15. Rapid directed molecular evolution of fluorescent proteins in mammalian cells.Protein science : a publication of the Protein Society · 2022
    Article
  16. Article
  17. Review
  18. Review
  19. ZnProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  20. Tools and techniques for illuminating the cell biology of zinc.Biochimica et biophysica acta. Molecular cell research · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Brett L FiedlerJILA, University of Colorado and NIST, Boulder Colorado 80309, United States.ORCID 0000-0002-1729-0185
Steven Van BuskirkJILA, University of Colorado and NIST, Boulder Colorado 80309, United States.
Kyle P CarterDepartment of Chemistry and Biochemistry, University of Colorado , Boulder Colorado 80309, United States.
Yan QinDepartment of Chemistry and Biochemistry, University of Colorado , Boulder Colorado 80309, United States.
Margaret C CarpenterDepartment of Chemistry and Biochemistry, University of Colorado , Boulder Colorado 80309, United States.
Amy E PalmerDepartment of Chemistry and Biochemistry, University of Colorado , Boulder Colorado 80309, United States.
Ralph JimenezJILA, University of Colorado and NIST, Boulder Colorado 80309, United States.ORCID 0000-0002-8989-405X

Funding

Regulation of Cell Signaling by Transition Metal DynamicsDP1GM114863 · NIGMS · UNIVERSITY OF COLORADO · PI PALMER, AMY E · 2014 to 2018
$3.8M
LEADERSHIP TRAINING IN PHARMACEUTICAL BIOTECHNOLOGYT32GM008732 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI RANDOLPH, THEODORE W · 2001 to 2015
$2.7M
Genetically Encoded Sensors Shed Light on Zinc HomeostasisR01GM084027 · NIGMS · UNIVERSITY OF COLORADO · PI PALMER, AMY E · 2008 to 2016
$2.6M
Generation of Cell-based and Animal-based Imaging Systems for Monitoring SynapticR00EB017289 · NIBIB · UNIVERSITY OF DENVER (COLORADO SEMINARY) · PI QIN, YAN · 2016 to 2018
$660k
Generation of Cell-based and Animal-based Imaging Systems for Monitoring SynapticK99EB017289 · NIBIB · UNIVERSITY OF COLORADO · PI QIN, YAN · 2014 to 2015
$97k
NIBIB NIH HHS R00 EB017289NIGMS NIH HHS DP1 GM114863NIGMS NIH HHS R01 GM084027NIGMS NIH HHS T32 GM008732
6 · The paper itself

Abstract

Fluorescent biosensors are important measurement tools for in vivo quantification of pH, concentrations of metal ions and other analytes, and physical parameters such as membrane potential. Both the development of these sensors and their implementation in examining cellular heterogeneity requires technology for measuring and sorting cells based on the fluorescence levels before and after chemical or physical perturbations. We developed a droplet microfluidic platform for the screening and separation of cell populations on the basis of the in vivo response of expressed fluorescence-based biosensors after addition of an exogenous analyte. We demonstrate the capability to resolve the responses of two genetically encoded Zn

Indexed as

Biosensing TechniquesFlow CytometryFluorescence Resonance Energy TransferMicrofluidic Analytical TechniquesFluorescenceFluorescent DyesHeLa CellsHumansParticle SizeRecombinant Fusion ProteinsZincFluorescent DyesRecombinant Fusion ProteinsZapCV2ZapCV5Zinc

Identifiers

PMID27959493
PMCPMC6019271

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.