ArticleRapid communications in mass spectrometry : RCM2017
Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies.
Article in Rapid communications in mass spectrometry : RCM, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Method Comparison of Dried Blood Spot and Plasma Apolipoprotein B Measurement on a Semi-Automated Analyser: Toward Accessible Lipid Profiling in Resource-Limited Settings.Analytical science advances · 2026Article
- Translational lipidomics reveals BMP and its precursor LPG as biomarkers for CLN5 Batten disease.bioRxiv : the preprint server for biology · 2026Article
- LC-MS-Based Global Metabolic Profiles of Alternative Blood Specimens Collected by Microsampling.Metabolites · 2025Article
- Investigation into temporal changes in the human bloodstain lipidome.International journal of legal medicine · 2025Article
- Comparison of maternal venous blood metabolomics collected as dried blood spots, dried blood microsamplers, and plasma for integrative environmental health research.Environment international · 2024Article
- Effects of storage temperature and time on metabolite profiles measured in dried blood spots, dried blood microsamplers, and plasma.The Science of the total environment · 2024Article
- A global metabolomics minefield: Confounding effects of preanalytical factors when studying rare disorders.Analytical science advances · 2023Article
- Dried blood spots in clinical lipidomics: optimization and recent findings.Analytical and bioanalytical chemistry · 2022Review
- Review
- Nontargeted mass spectrometry of dried blood spots for interrogation of the human circulating metabolome.Journal of mass spectrometry : JMS · 2021Article
- Volumetric Absorptive Microsampling of Blood for Untargeted Lipidomics.Molecules (Basel, Switzerland) · 2021Article
- Predicting human health from biofluid-based metabolomics using machine learning.Scientific reports · 2020Article
- Integrating Two-Dimensional Gas and Liquid Chromatography-Mass Spectrometry for Untargeted Colorectal Cancer Metabolomics: A Proof-of-Principle Study.Metabolites · 2020Article
- Combining lipidomics and machine learning to measure clinical lipids in dried blood spots.Metabolomics : Official journal of the Metabolomic Society · 2020Article
- Statistically Driven Metabolite and Lipid Profiling of Patients from the Undiagnosed Diseases Network.Analytical chemistry · 2020Article
- Evaluation of Dried Blood Spot Sampling for Clinical Metabolomics: Effects of Different Papers and Sample Storage Stability.Metabolites · 2019Article
- Metabolomics of Eothenomys miletus from five Hengduan Mountains locations in summer.Scientific reports · 2019Article
- Global Plasma Profiling for Colorectal Cancer-Associated Volatile Organic Compounds: a Proof-of-Principle Study.Journal of chromatographic science · 2019Article
- Dried Blood Spots for Global Health Diagnostics and Surveillance: Opportunities and Challenges.The American journal of tropical medicine and hygiene · 2018Review
- Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry.Bioanalysis · 2018Article
Corrections and comments
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Authors and funding
18 authors.
Funding
Abstract
rationaleThe use of dried blood spots (DBS) has many advantages over traditional plasma and serum samples such as the smaller blood volume required, storage at room temperature, and ability to sample in remote locations. However, understanding the robustness of different analytes in DBS samples is essential, especially in older samples collected for longitudinal studies.
methodsHere we analyzed the stability of polar metabolites and lipids in DBS samples collected in 2000-2001 and stored at room temperature. The identified and statistically significant molecules were then compared to matched serum samples stored at -80°C to determine if the DBS samples could be effectively used in a longitudinal study following metabolic disease.
resultsA total of 400 polar metabolites and lipids were identified in the serum and DBS samples using gas chromatograph/mass spectrometry (GC/MS), liquid chromatography (LC)/MS, and LC/ion mobility spectrometry-MS (LC/IMS-MS). The identified polar metabolites overlapped well between the sample types, though only one statistically significant metabolite was conserved in a case-control study of older diabetic males with low amounts of high-density lipoproteins and high body mass indices, triacylglycerides and glucose levels when compared to non-diabetic patients with normal levels, indicating that degradation in the DBS samples affects polar metabolite quantitation. Differences in the lipid identifications indicated that some oxidation occurs in the DBS samples. However, 36 statistically significant lipids correlated in both sample types.
conclusionsThe difference in the number of statistically significant polar metabolites and lipids indicated that the lipids did not degrade to as great of a degree as the polar metabolites in the DBS samples and lipid quantitation was still possible. Copyright © 2016 John Wiley & Sons, Ltd.
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