ArticleScientific reports2016
Highly efficient transduction of primary adult CNS and PNS neurons.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Bidirectional coupling of neuronal CaRedox biology · 2026Article
- Early to Late VSV-G Expression in AcMNPV BV Enhances Transduction in Mammalian Cells but Does Not Affect Virion Yield in Insect Cells.Vaccines · 2025Article
- Targeting Vasohibins to Promote Axon Regeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Inhibition of microtubule detyrosination by parthenolide facilitates functional CNS axon regeneration.eLife · 2023Article
- TUDCA protects against tunicamycin-induced apoptosis of dorsal root ganglion neurons by suppressing activation of ER stress.Experimental and therapeutic medicine · 2022Article
- Two photon imaging of calcium responses in murine Purkinje neurons.STAR protocols · 2022Article
- Autophagy and post-ischemic conditioning in retinal ischemia.Autophagy · 2021Article
- CXCR4/CXCL12-mediated entrapment of axons at the injury site compromises optic nerve regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Transneuronal delivery of hyper-interleukin-6 enables functional recovery after severe spinal cord injury in mice.Nature communications · 2021Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Delivery and expression of recombinant genes, a key methodology for many applications in biological research, remains a challenge especially for mature neurons. Here, we report easy, highly efficient and well tolerated transduction of adult peripheral and central neuronal populations of diverse species in culture using VSV-G pseudo-typed, recombinant baculovirus (BacMam). Transduction rates of up to 80% were reliably achieved at high multiplicity of infection without apparent neuro-cytopathic effects. Neurons could be transduced either shortly after plating or after several days in culture. Co-incubation with two different baculoviruses attained near complete co-localization of fluorescent protein expression, indicating multigene delivery. Finally, evidence for functional protein expression is provided by means of cre-mediated genetic recombination and neurite outgrowth assays. Recombinant protein was already detected within hours after transduction, thereby enabling functional readouts even in relatively short-lived neuronal cultures. Altogether, these results substantiate the usefulness of baculovirus-mediated transduction of mature neurons for future research in neuroscience.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.