ArticleScientific reports2016
General calibration of microbial growth in microplate readers.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 168 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
168 citing papers in PubMed, 1 synthesis or guideline pooled it, 354 citations in OpenAlex.
- Analysing and meta-analysing time-series data of microbial growth and gene expression from plate readers.PLoS computational biology · 2022Pooled it
- Stress Tolerance Assays for Salmonella enterica: Methods for Evaluating Heat, Acidity, and Osmotic Resistance.Methods in molecular biology (Clifton, N.J.) · 2027Article
- MXene-Based MnZnO Nanocomposites for Enhanced Antibacterial Activity and Cutaneous Wound Healing.Applied biochemistry and biotechnology · 2026Article
- Quantifying antibiotic susceptibility and inoculum effects using transient dynamics of Pseudomonas aeruginosa.PLoS computational biology · 2026Article
- Molecular and Functional Characterization of Black Soldier Fly Derived Peptide Against Food-Relevant Bacteria.Foods (Basel, Switzerland) · 2026Article
- Who wins? Analysis and Simulation of a Batch Culture Model of Bacterial Competition in the Presence of Plasmids.Bulletin of mathematical biology · 2026Article
- Contributions of intra- and extracellular antibiotic degradation to collective [Formula: see text]-lactam survival.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Microbial biodegradation of polypropylene microplastics: a comparative assessment of single- and dual-species models.Biodegradation · 2026Article
- Enterococcus faecalis NBRC 100481 Protects the Intestinal Barrier via α-catenin/HMP-1 in Caenorhabditis elegans.Probiotics and antimicrobial proteins · 2026Article
- A coma pattern-based autofocusing method resolves bacterial cold shock response at single-cell level.eLife · 2026Article
- A bacterial ally for nitrogen-fixing biofilm: enhancing the rhizosphere colonization ofApplied and environmental microbiology · 2026Article
- In Vitro Evaluation of Olorofim and Amphotericin B Combination Therapy AgainstJournal of fungi (Basel, Switzerland) · 2026Article
- RodA promotes intestinal colonization by group BInfection and immunity · 2026Article
- An Agar-Water-Assisted ODJournal of fungi (Basel, Switzerland) · 2026Article
- CApEsid biOfilm: a suggested pipeline for clinical phage microbiology for biofilm infections based on comparative method study.Scientific reports · 2026Article
- Cold atmospheric plasma for bacterial inactivation in Nile water and wastewater.Scientific reports · 2026Article
- Atmospheric Cold Microwave Argon Plasma for Decontamination of Dental Implant Surfaces: An In Vitro Experimental Study.Journal of functional biomaterials · 2026Article
- In Vitro Inhibition of Pathogens by Polyols: Optical Density-Based Screening and Implications for the Oral-Systemic Axis.Microorganisms · 2026Article
- Bacterial killing assays in ecoimmunology require cross-validation by agreement statistics.Biology open · 2026Article
- Inferring bacterial cell size dynamics across media conditions.Scientific reports · 2026Article
108 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Optical density (OD) measurements of microbial growth are one of the most common techniques used in microbiology, with applications ranging from studies of antibiotic efficacy to investigations of growth under different nutritional or stress environments, to characterization of different mutant strains, including those harbouring synthetic circuits. OD measurements are performed under the assumption that the OD value obtained is proportional to the cell number, i.e. the concentration of the sample. However, the assumption holds true in a limited range of conditions, and calibration techniques that determine that range are currently missing. Here we present a set of calibration procedures and considerations that are necessary to successfully estimate the cell concentration from OD measurements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.