ArticleInternational journal of nanomedicine
Enhancing the specificity of polymerase chain reaction by graphene oxide through surface modification: zwitterionic polymer is superior to other polymers with different charges.
Article in International journal of nanomedicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 27 citations in OpenAlex.
- The Evolution of Nucleic Acid-Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Application of Nanomaterials to Enhance Polymerase Chain Reaction.Molecules (Basel, Switzerland) · 2022Review
- Highly Specific Loop-Mediated Isothermal Amplification Using Graphene Oxide-Gold Nanoparticles Nanocomposite for Foot-and-Mouth Disease Virus Detection.Nanomaterials (Basel, Switzerland) · 2022Article
- Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR.Scientific reports · 2021Article
- Effects of graphene oxide on PCR amplification for microbial community survey.BMC microbiology · 2020Article
- Effect of green GO/Au nanocomposite on in-vitro amplification of human DNA.IET nanobiotechnology · 2019Article
- An Alternative Hot Start PCR Method Using a Nuclease-Deficient ExoIII from Escherichia coli.Molecular biotechnology · 2019Article
- Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers.Scientific reports · 2018Article
- Development of nanoparticle-assisted PCR assay in the rapid detection of brain-eating amoebae.Parasitology research · 2018Article
- Graphene oxide enhances the specificity of the polymerase chain reaction by modifying primer-template matching.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Graphene oxides (GOs) with different surface characteristics, such as size, reduction degree and charge, are prepared, and their effects on the specificity of polymerase chain reaction (PCR) are investigated. In this study, we demonstrate that GO with a large size and high reduction degree is superior to small and nonreduced GO in enhancing the specificity of PCR. Negatively charged polyacrylic acid (PAA), positively charged polyacrylamide (PAM), neutral polyethylene glycol (PEG) and zwitterionic polymer poly(sulfobetaine) (pSB) are used to modify GO. The PCR specificity-enhancing ability increases in the following order: GO-PAA < GO-PAM < GO-PEG < GO-pSB. Thus, zwitterionic polymer-modified GO is superior to other GO derivatives with different charges in enhancing the specificity of PCR. GO derivatives are also successfully used to enhance the specificity of PCR for the amplification of human mitochondrial DNA using blood genomic DNA as template. Molecular dynamics simulations and molecular docking are performed to elucidate the interaction between the polymers and
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