ArticleProceedings of the National Academy of Sciences of the United States of America2016
DNA demethylation is initiated in the central cells of Arabidopsis and rice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers, 1 of them a synthesis that pooled it.
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106 citing papers in PubMed, 1 synthesis or guideline pooled it, 202 citations in OpenAlex.
- Meta-analysis of RNA-Seq studies reveals genes with dominant functions during flower bud endo- to eco-dormancy transition in Prunus species.Scientific reports · 2021Pooled it
- Evolution of Epigenetic Regulation in Plant Reproduction.Epigenomes · 2026Review
- Regulatory insights underlying apple to cold stress.Horticulture research · 2026Article
- Integrated methylome and transcriptome analysis provides insight into DNA methylation-mediated networks in sexual dimorphism of Vernicia montana.BMC plant biology · 2026Article
- From the MMC Specification to Endosperm Cellularization in Arabidopsis: A Developmental-Handover Framework for Seed Initiation.Plants (Basel, Switzerland) · 2026Review
- Transposable element-mediated DNA methylation of the NAC20 and NAC26 promoters led to a maternal effect on grain filling.Journal of integrative plant biology · 2026Article
- Imprinting and DNA Methylation in Water Lily Endosperm: Implications for Seed Evolution.Molecular biology and evolution · 2025Article
- The 5-methylcytosine DNA glycosylase ROS1 prevents paternal genome hypermethylation in Arabidopsis endosperm.Genome biology · 2025Article
- Article
- MDR1 DNA glycosylase regulates the expression of genomically imprinted genes and Helitrons.The Plant journal : for cell and molecular biology · 2025Article
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- The Expansion and Diversification of Epigenetic Regulatory Networks Underpins Major Transitions in the Evolution of Land Plants.Molecular biology and evolution · 2025Article
- DNA methylation dynamics in male germline development in Brassica Rapa.Molecular horticulture · 2025Article
- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- Using Callus as an Ex Vivo System for Chromatin Analysis.Methods in molecular biology (Clifton, N.J.) · 2025Review
- DEMETER DNA demethylase reshapes the global DNA methylation landscape and controls cell identity transition during plant regeneration.BMC genomics · 2024Article
- Parental dialectic: Epigenetic conversations in endosperm.Current opinion in plant biology · 2024Review
- Potent pollen gene regulation by DNA glycosylases in maize.Nature communications · 2024Article
- Molecular basis and evolutionary drivers of endosperm-based hybridization barriers.Plant physiology · 2024Review
- DNA methylation remodeling and the functional implication during male gametogenesis in rice.Genome biology · 2024Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 5 institutions in 5 countries.
Funding
Abstract
Cytosine methylation is a DNA modification with important regulatory functions in eukaryotes. In flowering plants, sexual reproduction is accompanied by extensive DNA demethylation, which is required for proper gene expression in the endosperm, a nutritive extraembryonic seed tissue. Endosperm arises from a fusion of a sperm cell carried in the pollen and a female central cell. Endosperm DNA demethylation is observed specifically on the chromosomes inherited from the central cell in Arabidopsis thaliana, rice, and maize, and requires the DEMETER DNA demethylase in Arabidopsis DEMETER is expressed in the central cell before fertilization, suggesting that endosperm demethylation patterns are inherited from the central cell. Down-regulation of the MET1 DNA methyltransferase has also been proposed to contribute to central cell demethylation. However, with the exception of three maize genes, central cell DNA methylation has not been directly measured, leaving the origin and mechanism of endosperm demethylation uncertain. Here, we report genome-wide analysis of DNA methylation in the central cells of Arabidopsis and rice-species that diverged 150 million years ago-as well as in rice egg cells. We find that DNA demethylation in both species is initiated in central cells, which requires DEMETER in Arabidopsis However, we do not observe a global reduction of CG methylation that would be indicative of lowered MET1 activity; on the contrary, CG methylation efficiency is elevated in female gametes compared with nonsexual tissues. Our results demonstrate that locus-specific, active DNA demethylation in the central cell is the origin of maternal chromosome hypomethylation in the endosperm.
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