ArticleProceedings of the National Academy of Sciences of the United States of America2016
Pathway swapping: Toward modular engineering of essential cellular processes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- Synthetic Chromosomes in Yeast: A Platform for Understanding and Programming Eukaryotic Life.Microbial biotechnology · 2026Review
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Article
- Life beyond 6000 genes: from sequence to synthesis in a post-genomics era.FEMS yeast research · 2026Review
- Article
- A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Genetic Engineering and Synthetic Genomics in Yeast to Understand Life and Boost Biotechnology.Bioengineering (Basel, Switzerland) · 2020Review
- Combining mechanistic and machine learning models for predictive engineering and optimization of tryptophan metabolism.Nature communications · 2020Article
- Design and Experimental Evaluation of a Minimal, Innocuous Watermarking Strategy to Distinguish Near-Identical DNA and RNA Sequences.ACS synthetic biology · 2020Article
- Adaptive Laboratory Evolution and Reverse Engineering of Single-Vitamin Prototrophies in Saccharomyces cerevisiae.Applied and environmental microbiology · 2020Article
- Phylogenetic debugging of a complete human biosynthetic pathway transplanted into yeast.Nucleic acids research · 2020Article
- Rewired cellular signaling coordinates sugar and hypoxic responses for anaerobic xylose fermentation in yeast.PLoS genetics · 2019Article
- Allele-specific genome editing using CRISPR-Cas9 is associated with loss of heterozygosity in diploid yeast.Nucleic acids research · 2019Article
- Reassessment of requirements for anaerobic xylose fermentation by engineered, non-evolved Saccharomyces cerevisiae strains.FEMS yeast research · 2019Article
- Structural, Physiological and Regulatory Analysis of Maltose Transporter Genes inFrontiers in microbiology · 2018Article
- The Genetic Makeup and Expression of the Glycolytic and Fermentative Pathways Are Highly Conserved Within theFrontiers in genetics · 2018Article
- Selection of PofFrontiers in microbiology · 2018Article
- TheBiotechnology for biofuels · 2018Article
- Synthesis of Programmable Bioelectronic Yeast for Biohybrid Futures.Engineering biologyReview
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9 authors.
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Abstract
Recent developments in synthetic biology enable one-step implementation of entire metabolic pathways in industrial microorganisms. A similarly radical remodelling of central metabolism could greatly accelerate fundamental and applied research, but is impeded by the mosaic organization of microbial genomes. To eliminate this limitation, we propose and explore the concept of "pathway swapping," using yeast glycolysis as the experimental model. Construction of a "single-locus glycolysis" Saccharomyces cerevisiae platform enabled quick and easy replacement of this yeast's entire complement of 26 glycolytic isoenzymes by any alternative, functional glycolytic pathway configuration. The potential of this approach was demonstrated by the construction and characterization of S. cerevisiae strains whose growth depended on two nonnative glycolytic pathways: a complete glycolysis from the related yeast Saccharomyces kudriavzevii and a mosaic glycolysis consisting of yeast and human enzymes. This work demonstrates the feasibility and potential of modular, combinatorial approaches to engineering and analysis of core cellular processes.
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