ArticleThe Journal of biological chemistry2017
Catalytic Mechanism of a Novel Glycoside Hydrolase Family 16 "Elongating" β-Transglycosylase.
Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Development of an Immunoassay Platform Targeting β-1,3- and β-1,6-Glucans for Rapid Detection of Fungi.Journal of fungi (Basel, Switzerland) · 2026Article
- An Investigation of the Secretome Composition ofInternational journal of molecular sciences · 2025Article
- Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors.Waste and biomass valorization · 2023Article
- The Maize Pathogen Ustilago maydis Secretes Glycoside Hydrolases and Carbohydrate Oxidases Directed toward Components of the Fungal Cell Wall.Applied and environmental microbiology · 2022Article
- Highlighting the factors governing transglycosylation in the GH5_5 endo-1,4-β-glucanase RBcel1.Acta crystallographica. Section D, Structural biology · 2022Article
- Molecular Basis for Substrate Recognition and Catalysis by a Marine Bacterial Laminarinase.Applied and environmental microbiology · 2020Article
- Article
- A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.Molecules (Basel, Switzerland) · 2020Article
- A subfamily roadmap of the evolutionarily diverse glycoside hydrolase family 16 (GH16).The Journal of biological chemistry · 2019Article
- Insights into the dual cleavage activity of the GH16 laminarinase enzyme class on β-1,3 and β-1,4 glycosidic bonds.The Journal of biological chemistryArticle
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Authors and funding
6 authors.
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Abstract
Carbohydrates are complex macromolecules in biological metabolism. Enzymatic synthesis of carbohydrates is recognized as a powerful tool to overcome the problems associated with large scale synthesis of carbohydrates. Novel enzymes with significant transglycosylation ability are still in great demand in glycobiology studies. Here we report a novel glycoside hydrolase family 16 "elongating" β-transglycosylase from Paecilomyces thermophila (PtBgt16A), which efficiently catalyzes the synthesis of higher polymeric oligosaccharides using β-1,3/1,4-oligosaccharides as donor/acceptor substrates. Further structural information reveals that PtBgt16A has a binding pocket around the -1 subsite. The catalytic mechanism of PtBgt16A is partly similar to an exo-glycoside hydrolase, which cleaves the substrate from the non-reducing end one by one. However, PtBgt16A releases the reducing end product and uses the remainder glucosyl as a transglycosylation donor. This catalytic mechanism has similarity with the catalytic mode of amylosucrase, which catalyzes the transglycosylation products gradually extend by one glucose unit. PtBgt16A thus has the potential to be a tool enzyme for the enzymatic synthesis of new β-oligosaccharides and glycoconjugates.
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