ArticlePlant physiology2017
Physical Mapping of Amplified Copies of the 5-Enolpyruvylshikimate-3-Phosphate Synthase Gene in Glyphosate-Resistant Amaranthus tuberculatus.
Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- Aneusomaty in glyphosate-resistant Amaranthus palmeri and its hybrid with Amaranthus tuberculatus.Genes & genomics · 2025Article
- Appearance of transient heteromorphic large chromosome in glyphosate-resistant Amaranthus tuberculatus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate.The Plant cell · 2025Article
- Extrachromosomal circular DNA-mediated spread of herbicide resistance in interspecific hybrids of pigweed.Plant physiology · 2023Article
- Article
- Multiple evolutionary origins of glyphosate resistance inEvolutionary applications · 2022Article
- Expression Pattern of Entire Cytochrome P450 Genes and Response of Defenses in a Metabolic-Herbicide-Resistant Biotype ofFrontiers in plant science · 2022Article
- Evolution of Glyphosate-Resistant Weeds.Reviews of environmental contamination and toxicology · 2021Review
- Fitness Cost Associated With EnhancedFrontiers in plant science · 2021Article
- Ribosomal DNA localization on Lathyrus species chromosomes by FISH.Journal, genetic engineering & biotechnology · 2020Article
- Increased Glyphosate-Induced Gene Expression in the Shikimate Pathway Is Abolished in the Presence of Aromatic Amino Acids and Mimicked by Shikimate.Frontiers in plant science · 2020Article
- Physiological performance of glyphosate and imazamox mixtures on Amaranthus palmeri sensitive and resistant to glyphosate.Scientific reports · 2019Article
- Molecular cloning and metabolomic characterization of the 5-enolpyruvylshikimate-3-phosphate synthase gene from Baphicacanthus cusia.BMC plant biology · 2019Article
- Multiple modes of convergent adaptation in the spread of glyphosate-resistantProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Fluorescence in situ hybridization in plants: recent developments and future applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Review
- Selective fertilization with phosphite allows unhindered growth of cotton plants expressing theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Evaluation of glyphosate resistance in Arabidopsis thaliana expressing an altered target site EPSPS.Pest management science · 2018Article
- Extrachromosomal circular DNA-based amplification and transmission of herbicide resistance in crop weedProceedings of the National Academy of Sciences of the United States of America · 2018Article
- A Novel Role of Ring Chromosomes as Evolutionary Drivers of Herbicide Resistance.Plant physiology · 2018Article
- Gene Duplication and Aneuploidy Trigger Rapid Evolution of Herbicide Resistance in Common Waterhemp.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent and rapid evolution of resistance to glyphosate, the most widely used herbicides, in several weed species, including common waterhemp (Amaranthus tuberculatus), poses a serious threat to sustained crop production. We report that glyphosate resistance in A tuberculatus was due to amplification of the 5-enolpyruvylshikimate-3-P synthase (EPSPS) gene, which encodes the molecular target of glyphosate. There was a positive correlation between EPSPS gene copies and its transcript expression. We analyzed the distribution of EPSPS copies in the genome of A tuberculatus using fluorescence in situ hybridization on mitotic metaphase chromosomes and interphase nuclei. Fluorescence in situ hybridization analysis mapped the EPSPS gene to pericentromeric regions of two homologous chromosomes in glyphosate sensitive A tuberculatus In glyphosate-resistant plants, a cluster of EPSPS genes on the pericentromeric region on one pair of homologous chromosomes was detected. Intriguingly, two highly glyphosate-resistant plants harbored an additional chromosome with several EPSPS copies besides the native chromosome pair with EPSPS copies. These results suggest that the initial event of EPSPS gene duplication may have occurred because of unequal recombination mediated by repetitive DNA. Subsequently, gene amplification may have resulted via several other mechanisms, such as chromosomal rearrangements, deletion/insertion, transposon-mediated dispersion, or possibly by interspecific hybridization. This report illustrates the physical mapping of amplified EPSPS copies in A tuberculatus.
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