ArticleJournal of tissue engineering and regenerative medicine2018
Re-endothelialization of rat lung scaffolds through passive, gravity-driven seeding of segment-specific pulmonary endothelial cells.
Article in Journal of tissue engineering and regenerative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Targeted cell deposition for lung re-endothelialization.Biomechanics and modeling in mechanobiology · 2026Article
- Decellularized Rat Lung Extracellular Matrix as an In Vitro Platform for Canine Yolk Sac-Derived Endothelial Precursor Cells for Pulmonary Endothelium Reconstruction Studies.Bioengineering (Basel, Switzerland) · 2026Article
- Computer vision to predict cell seeding coverage in re-endothelialized mouse lungs.Scientific reports · 2025Article
- Two Decades of Advances and Limitations in Organ Recellularization.Current issues in molecular biology · 2024Review
- Orthotopic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells.Scientific reports · 2024Article
- Lung endothelium, tau, and amyloids in health and disease.Physiological reviews · 2024Review
- Bioengineering and Clinical Translation of Human Lung and its Components.Advanced biology · 2023Review
- Vascular reconstruction of the decellularized biomatrix for whole-organ engineering-a critical perspective and future strategies.Frontiers in bioengineering and biotechnology · 2023Review
- Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Lung Tissue Engineering: Toward a More Deliberate Approach.ACS biomaterials science & engineering · 2022Review
- Bioactive Cell-Derived ECM Scaffold Forms a Unique Cellular Microenvironment for Lung Tissue Engineering.Biomedicines · 2022Article
- Emerging Paradigms in Bioengineering the Lungs.Bioengineering (Basel, Switzerland) · 2022Review
- Cell Inertia: Predicting Cell Distributions in Lung Vasculature to Optimize Re-endothelialization.Frontiers in bioengineering and biotechnology · 2022Article
- The Potential Contribution of Biopolymeric Particles in Lung Tissue Regeneration of COVID-19 Patients.Polymers · 2021Review
- Building a stem cell-based primate uterus.Communications biology · 2021Review
- Engineering Functional Vasculature in Decellularized Lungs Depends on Comprehensive Endothelial Cell Tropism.Frontiers in bioengineering and biotechnology · 2021Article
- Acellular Biologic Nipple-Areolar Complex Graft:Tissue engineering. Part A · 2020Article
- Human-scale lung regeneration based on decellularized matrix scaffolds as a biologic platform.Surgery today · 2020Review
- Bioengineering the Blood-gas Barrier.Comprehensive Physiology · 2020Review
- Lung Microvascular Niche, Repair, and Engineering.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Effective re-endothelialization is critical for the use of decellularized scaffolds for ex vivo lung engineering. Current approaches yield insufficiently re-endothelialized scaffolds that haemorrhage and become thrombogenic upon implantation. Herein, gravity-driven seeding coupled with bioreactor culture facilitated widespread distribution and engraftment of endothelial cells throughout rat lung scaffolds. Initially, human umbilical vein endothelial cells were seeded into the pulmonary artery by either gravity-driven, variable flow perfusion seeding or pump-driven, pulsatile flow perfusion seeding. Gravity seeding evenly distributed cells and supported cell survival and re-lining of the vascular walls while perfusion pump-driven seeding led to increased cell fragmentation and death. Using gravity seeding, rat pulmonary artery endothelial cells and rat pulmonary vein endothelial cells attached in intermediate and large vessels, while rat pulmonary microvascular endothelial cells deposited mostly in microvessels. Combination seeding of these cells led to positive vascular endothelial cadherin staining. In addition, combination seeding improved barrier function as assessed by serum albumin extravasation; however, leakage was observed in the distal portions of the re-endothelialized tissue suggesting that recellularization of the alveoli is necessary to complete barrier function of the capillary-alveolar network. Overall, these data indicate that vascular recellularization of rat lung scaffolds is achieved through gravity seeding. Copyright © 2016 John Wiley & Sons, Ltd.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.