ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2017
An innovative SNP genotyping method adapting to multiple platforms and throughputs.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 126 citations in OpenAlex.
- Two complementary NLRs from wild emmer wheat confer powdery mildew resistance.Nature communications · 2025Article
- Genetic analysis of the single internode dwarf 1 mutant in barley.BMC plant biology · 2025Article
- Development of diagnostic markers for the disease susceptibility gene Tsn1 in wheat reveals novel resistance alleles and a new locus required for ToxA sensitivity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Genetic and physical localization of a leaf rust susceptibility gene in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Construction of a genetic linkage map and QTL mapping of the agronomic traits in Foxtail millet (Setaria italica).BMC genomics · 2025Article
- Identification of genetic loci and candidate genes underlying Fusarium crown rot resistance in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- An Assay for Variable Fragment Length Allele-Specific Genotyping.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A novel strategy to map a locus associated with flowering time in canola (Brassica napus L.).Molecular genetics and genomics : MGG · 2024Article
- Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- New Insights on Tools for Detecting β-Tubulin Polymorphisms inAnimals : an open access journal from MDPI · 2024Article
- The Hessian fly resistance gene HvRHF1 is localized in an NBS-LRR gene cluster in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- A novel QTL conferring Fusarium crown rot resistance on chromosome 2A in a wheat EMS mutant.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Identification of Sr67, a new gene for stem rust resistance in KU168-2 located close to the Sr13 locus in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Enrichment and Diversification of the Wheat Genome via Alien Introgression.Plants (Basel, Switzerland) · 2024Review
- Linkage drag analysis in three Aegilops speltoides introgressions carrying Sr47 in modern durum and hard red spring wheat germplasm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Whole-genome sequencing enables molecular dissection and candidate gene identification of the rust resistance gene RTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Genetic and physical localization of a major susceptibility gene to Pyrenophora teres f. maculata in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- CRISPR-induced miRNA156-recognition element mutations in TaSPL13 improve multiple agronomic traits in wheat.Plant biotechnology journal · 2023Article
- Genetic Insight into Disease Resistance Gene Clusters by Using Sequencing-Based Fine Mapping in Sunflower (International journal of molecular sciences · 2022Article
- Haplotype variants of Sr46 in Aegilops tauschii, the diploid D genome progenitor of wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageAn innovative genotyping method designated as semi-thermal asymmetric reverse PCR (STARP) was developed for genotyping individual SNPs with improved accuracy, flexible throughputs, low operational costs, and high platform compatibility. Multiplex chip-based technology for genome-scale genotyping of single nucleotide polymorphisms (SNPs) has made great progress in the past two decades. However, PCR-based genotyping of individual SNPs still remains problematic in accuracy, throughput, simplicity, and/or operational costs as well as the compatibility with multiple platforms. Here, we report a novel SNP genotyping method designated semi-thermal asymmetric reverse PCR (STARP). In this method, genotyping assay was performed under unique PCR conditions using two universal priming element-adjustable primers (PEA-primers) and one group of three locus-specific primers: two asymmetrically modified allele-specific primers (AMAS-primers) and their common reverse primer. The two AMAS-primers each were substituted one base in different positions at their 3' regions to significantly increase the amplification specificity of the two alleles and tailed at 5' ends to provide priming sites for PEA-primers. The two PEA-primers were developed for common use in all genotyping assays to stringently target the PCR fragments generated by the two AMAS-primers with similar PCR efficiencies and for flexible detection using either gel-free fluorescence signals or gel-based size separation. The state-of-the-art primer design and unique PCR conditions endowed STARP with all the major advantages of high accuracy, flexible throughputs, simple assay design, low operational costs, and platform compatibility. In addition to SNPs, STARP can also be employed in genotyping of indels (insertion-deletion polymorphisms). As vast variations in DNA sequences are being unearthed by many genome sequencing projects and genotyping by sequencing, STARP will have wide applications across all biological organisms in agriculture, medicine, and forensics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.