ArticleEnvironmental sciences Europe2016
Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties.
Article in Environmental sciences Europe, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Genetically modified soybean lines exhibit less transcriptomic variation compared to natural varieties.GM crops & food · 2023Article
- Elevated COScientific reports · 2020Article
- Epigenomic landscape and epigenetic regulation in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Genetic and Global Epigenetic Modification, Which Determines the Phenotype of Transgenic Rice?International journal of molecular sciences · 2020Article
- Overexpression of a phospholipase (OsPLDα1) for drought tolerance in upland rice (Oryza sativa L.).Protoplasma · 2018Article
- Overexpression of SbSI-1, A Nuclear Protein fromFrontiers in plant science · 2017Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrior to their release in the environment, transgenic crops are examined for their health and environmental safety. In addition, transgene expression needs to be consistent in order to express the introduced trait (e.g. insecticidal and/or herbicide tolerance). Moreover, data on expression levels for GM events are usually required for approval, but these are rarely disclosed or they are considered insufficient. On the other hand, biosafety regulators do not consider epigenetic regulation (e.g. DNA methylation, ncRNAs and histone modifications), which are broadly known to affect gene expression, within their risk assessment analyses. Here we report the results of a DNA methylation (bisulfite sequencing) and transgene transcript accumulation (RT-qPCR) analysis of four Bt-expressing single transgenic maize hybrids, under different genetic backgrounds, and a stacked transgenic hybrid expressing both insecticidal and herbicide tolerance traits.
resultsOur results showed differences in cytosine methylation levels in the FMV promoter and
conclusionsOverall, our results show differences in DNA methylation patterns in all varieties, as well as in transgene transcript accumulation levels. Although the detection of changes in DNA methylation and transgenic accumulation levels does not present a safety issue per se, it demonstrates the need for additional studies that focus on detecting possible safety implications of such changes.
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