ArticlePLoS computational biology2016
Precision of Readout at the hunchback Gene: Analyzing Short Transcription Time Traces in Living Fly Embryos.
Article in PLoS computational biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
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- Bicoid-nucleosome competition sets a concentration threshold for transcription constrained by genome replication.Cell reports · 2025Article
- Optogenetic dissection of transcriptional repression in a multicellular organism.Nature communications · 2024Article
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- Fluctuating landscapes and heavy tails in animal behavior.bioRxiv : the preprint server for biology · 2023Article
- BurstDECONV: a signal deconvolution method to uncover mechanisms of transcriptional bursting in live cells.Nucleic acids research · 2023Article
- Transcriptional bursting: stochasticity in deterministic development.Development (Cambridge, England) · 2023Review
- Transcriptional activators in the early Drosophila embryo perform different kinetic roles.Cell systems · 2023Article
- Minimal synthetic enhancers reveal control of the probability of transcriptional engagement and its timing by a morphogen gradient.Cell systems · 2023Article
- Synthetic reconstruction of theeLife · 2022Article
- Quantitative imaging of transcription in living Drosophila embryos reveals the impact of core promoter motifs on promoter state dynamics.Nature communications · 2021Article
- Stochastic pausing at latent HIV-1 promoters generates transcriptional bursting.Nature communications · 2021Article
- Real-time single-cell characterization of the eukaryotic transcription cycle reveals correlations between RNA initiation, elongation, and cleavage.PLoS computational biology · 2021Article
- Investigating heterogeneities of live mesenchymal stromal cells using AI-based label-free imaging.Scientific reports · 2021Article
- The many bits of positional information.Development (Cambridge, England) · 2021Review
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- A matter of time: Using dynamics and theory to uncover mechanisms of transcriptional bursting.Current opinion in cell biology · 2020Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The simultaneous expression of the hunchback gene in the numerous nuclei of the developing fly embryo gives us a unique opportunity to study how transcription is regulated in living organisms. A recently developed MS2-MCP technique for imaging nascent messenger RNA in living Drosophila embryos allows us to quantify the dynamics of the developmental transcription process. The initial measurement of the morphogens by the hunchback promoter takes place during very short cell cycles, not only giving each nucleus little time for a precise readout, but also resulting in short time traces of transcription. Additionally, the relationship between the measured signal and the promoter state depends on the molecular design of the reporting probe. We develop an analysis approach based on tailor made autocorrelation functions that overcomes the short trace problems and quantifies the dynamics of transcription initiation. Based on live imaging data, we identify signatures of bursty transcription initiation from the hunchback promoter. We show that the precision of the expression of the hunchback gene to measure its position along the anterior-posterior axis is low both at the boundary and in the anterior even at cycle 13, suggesting additional post-transcriptional averaging mechanisms to provide the precision observed in fixed embryos.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.