ArticlePloS one2016
Lymph Node Transplantation Decreases Swelling and Restores Immune Responses in a Transgenic Model of Lymphedema.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Microsurgical techniques in the treatment of breast cancer-related lymphedema: a systematic review of efficacy and patient outcomes.Breast cancer (Tokyo, Japan) · 2021Pooled it
- Establishment of a long-term secondary lymphedema animal model in the rodent hindlimb.Frontiers in surgery · 2025Article
- Article
- Vascularized lymph node transplantation successfully reverses lymphedema and maintains immunity in a rat lymphedema model.Bioengineering & translational medicine · 2022Article
- Animal models in lymph node transfer surgery: A systematic review.Journal of clinical and translational research · 2022Review
- Pharmacological Treatment of Secondary Lymphedema.Frontiers in pharmacology · 2022Review
- Doxycycline for the treatment of breast cancer-related lymphedema.Frontiers in pharmacology · 2022Article
- Combined deep inferior epigastric artery perforator flap with vascularized groin lymph node transplant for treatment of breast cancer-related lymphedema.Gland surgery · 2021Review
- Mesenteric Lymph Node Transplantation in Mice to Study Immune Responses of the Gastrointestinal Tract.Frontiers in immunology · 2021Article
- IGCS Intraoperative Technology Taskforce. Update on near infrared imaging technology: beyond white light and the naked eye, indocyanine green and near infrared technology in the treatment of gynecologic cancers.International journal of gynecological cancer : official journal of the International Gynecological Cancer Society · 2020Review
- Histopathologic Features of Lymphedema: A Molecular Review.International journal of molecular sciences · 2020Review
- Lymph node stromal CCL2 limits antibody responses.Science immunology · 2020Article
- Controversies in Surgical Management of Lymphedema.Plastic and reconstructive surgery. Global open · 2020Article
- T helper 2 differentiation is necessary for development of lymphedema.Translational research : the journal of laboratory and clinical medicine · 2019Article
- Modulation of Immunity by Lymphatic Dysfunction in Lymphedema.Frontiers in immunology · 2019Review
- Combining Autologous Breast Reconstruction and Vascularized Lymph Node Transfer.Seminars in plastic surgery · 2018Review
- Vascularized Lymph Node Transfer for Lymphedema.Seminars in plastic surgery · 2018Review
- Free Vascularized Supraclavicular Autologous Heterotopic Lymph Node Transfers Without Skin Paddle for Lymphedema Lower Limb.International journal of applied & basic medical researchArticle
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 4 countries.
Funding
Abstract
introductionSecondary lymphedema is a common complication of cancer treatment and recent studies have demonstrated that lymph node transplantation (LNT) can decrease swelling, as well as the incidence of infections. However, although these results are exciting, the mechanisms by which LNT improves these pathologic findings of lymphedema remain unknown. Using a transgenic mouse model of lymphedema, this study sought to analyze the effect of LNT on lymphatic regeneration and T cell-mediated immune responses.
methodsWe used a mouse model in which the expression of the human diphtheria toxin receptor is driven by the FLT4 promoter to enable the local ablation of the lymphatic system through subdermal hindlimb diphtheria toxin injections. Popliteal lymph node dissection was subsequently performed after a two-week recovery period, followed by either orthotopic LNT or sham surgery after an additional two weeks. Hindlimb swelling, lymphatic vessel regeneration, immune cell trafficking, and T cell-mediated immune responses were analyzed 10 weeks later.
resultsLNT resulted in a marked decrease in hindlimb swelling, fibroadipose tissue deposition, and decreased accumulation of perilymphatic inflammatory cells, as compared to controls. In addition, LNT induced a marked lymphangiogenic response in both capillary and collecting lymphatic vessels. Interestingly, the resultant regenerated lymphatics were abnormal in appearance on lymphangiography, but LNT also led to a notable increase in dendritic cell trafficking from the periphery to the inguinal lymph nodes and improved adaptive immune responses.
conclusionsLNT decreases pathological changes of lymphedema and was shown to potently induce lymphangiogenesis. Lymphatic vessels induced by LNT were abnormal in appearance, but were functional and able to transport antigen-presenting cells. Animals treated with LNT have an increased ability to mount T cell-mediated immune responses when sensitized to antigens in the affected hindlimb.
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