ArticlePloS one2016
Validation of Reference Genes for Real-Time Quantitative PCR (qPCR) Analysis of Avibacterium paragallinarum.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Epidemiological Investigation of Infectious Coryza in Central China and the Effect of Enterococcus faecium on Improving Vaccine Immunity.Poultry science · 2025Article
- Development and evaluation of an attenuated Avibacterium paragallinarum strain as a live vaccine candidate for infectious coryza.Veterinary research · 2025Article
- The quadruplex fluorescent quantitative PCR method for the simultaneous detection of respiratory diseases in quail:Frontiers in microbiology · 2025Article
- Prevalence of NonpathogenicTransboundary and emerging diseases · 2025Article
- Selection and validation of reference genes suitable for gene expression analysis by Reverse Transcription Quantitative real-time PCR in Acinetobacter baumannii.Scientific reports · 2024Article
- The absence ofFrontiers in veterinary science · 2024Article
- Chemical Profile, Antibacterial, Antibiofilm, and Antiviral Activities ofMolecules (Basel, Switzerland) · 2023Article
- The Protective Efficacy of an Inactivated Vaccine againstVeterinary sciences · 2022Article
- Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes.Nucleic acids research · 2021Article
- The Transcriptomic and Bioinformatic Characterizations of Iron Acquisition and Heme Utilization inFrontiers in microbiology · 2021Article
- A Highly Sensitive and Specific Probe-Based Real-Time PCR for the Detection ofFrontiers in veterinary science · 2021Article
- A Novel Lateral Flow Assay for Rapid and Sensitive Nucleic Acid Detection ofFrontiers in veterinary science · 2021Article
- Article
- Cinnamon oil downregulates virulence genes of poultry respiratory bacterial agents and revealed significant bacterial inhibition: AnVeterinary world · 2019Article
- Comparative Genomic Analysis of Soil Dwelling Bacteria Utilizing a Combinational Codon Usage and Molecular Phylogenetic Approach Accentuating on Key Housekeeping Genes.Frontiers in microbiology · 2019Article
- Stable Reference Gene Selection for RT-qPCR Analysis inBioMed research international · 2019Article
- InnB, a Novel Type III Effector ofFrontiers in microbiology · 2018Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization Under Ethanol Stress Conditions inFrontiers in microbiology · 2018Article
- Comprehensive evaluation of blood-brain barrier-forming micro-vasculatures: Reference and marker genes with cellular composition.PloS one · 2018Article
- Validation of optimal reference genes for quantitative real time PCR in muscle and adipose tissue for obesity and diabetes research.Scientific reports · 2017Article
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4 authors.
Funding
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Abstract
Real-time quantitative reverse transcription PCR (qRT-PCR) offers a robust method for measurement of gene expression levels. Selection of reliable reference gene(s) for gene expression study is conducive to reduce variations derived from different amounts of RNA and cDNA, the efficiency of the reverse transcriptase or polymerase enzymes. Until now reference genes identified for other members of the family Pasteurellaceae have not been validated for Avibacterium paragallinarum. The aim of this study was to validate nine reference genes of serovars A, B, and C strains of A. paragallinarum in different growth phase by qRT-PCR. Three of the most widely used statistical algorithms, geNorm, NormFinder and ΔCT method were used to evaluate the expression stability of reference genes. Data analyzed by overall rankings showed that in exponential and stationary phase of serovar A, the most stable reference genes were gyrA and atpD respectively; in exponential and stationary phase of serovar B, the most stable reference genes were atpD and recN respectively; in exponential and stationary phase of serovar C, the most stable reference genes were rpoB and recN respectively. This study provides recommendations for stable endogenous control genes for use in further studies involving measurement of gene expression levels.
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