ArticleScientific reports2016
High fidelity visualization of cell-to-cell variation and temporal dynamics in nascent extracellular matrix formation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed.
- Nascent Extracellular Matrix Converts Biomaterial Cues into Cell Fate Decisions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Tracking Spatiotemporal Extracellular Matrix Evolution and Tissue Fusion in 3D Microtissues via Click Chemistry-Based Metabolic Labelling.Advanced healthcare materials · 2026Article
- Inhibition of fibroblast activation protein partially restores the homeostatic phenotype of mechanoactivated synoviocytes.Osteoarthritis and cartilage · 2026Article
- Nascent extracellular matrix: the missing piece in hydrogel design.Cell biomaterials · 2026Article
- Click Chemistry-Based Quantification of Extracellular Matrix Turnover for Drug Screening and Regenerative Medicine.Annals of biomedical engineering · 2026Article
- Adipose stromal cells in the human rotator cuff are resistant to fibrotic microenvironmental cues.The Journal of physiology · 2025Article
- Sex-stratified osteochondral organ-on-chip model reveals sex-specific responses to inflammatory stimulation.Materials today. Bio · 2025Article
- Development of the mechanoresponsive pericellular matrix of chondrons.Science advances · 2025Article
- High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC).Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Application of Tendon-Derived Matrix and Carbodiimide Crosslinking Matures the Engineered Tendon-Like Proteome on Meltblown Scaffolds.Journal of tissue engineering and regenerative medicine · 2025Article
- Rapid cellular uptake of citrate-coated iron oxide nanoparticles unaffected by cell-surface glycosaminoglycans.Nanoscale advances · 2024Article
- Microinterfaces in biopolymer-based bicontinuous hydrogels guide rapid 3D cell migration.Nature communications · 2024Article
- Modulating mechanobiology as a therapeutic target for synovial fibrosis to restore joint lubrication.Osteoarthritis and cartilage · 2024Article
- Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem-Cell Niches for In Vitro Models.Advanced materials (Deerfield Beach, Fla.) · 2023Review
- Unveiling extracellular matrix assembly: Insights and approaches through bioorthogonal chemistry.Materials today. Bio · 2023Review
- Mechanoepigenetic regulation of extracellular matrix homeostasis via Yap and Taz.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Pericellular heparan sulfate proteoglycans: Role in regulating the biosynthetic response of nucleus pulposus cells to osmotic loading.JOR spine · 2022Article
- Extracellular matrix dynamics: tracking in biological systems and their implications.Journal of biological engineering · 2022Review
- Metabolic labeling of secreted matrix to investigate cell-material interactions in tissue engineering and mechanobiology.Nature protocols · 2022Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Extracellular matrix dynamics are key to tissue morphogenesis, homeostasis, injury, and repair. The spatiotemporal organization of this matrix has profound biological implications, but is challenging to monitor using standard techniques. Here, we address these challenges by using noncanonical amino acid tagging to fluorescently label extracellular matrix synthesized in the presence of bio-orthogonal methionine analogs. This strategy labels matrix proteins with high resolution, without compromising their distribution or mechanical function. We demonstrate that the organization and temporal dynamics of the proteinaceous matrix depend on the biophysical features of the microenvironment, including the biomaterial scaffold and the niche constructed by cells themselves. Pulse labeling experiments reveal that, in immature constructs, nascent matrix is highly fibrous and interdigitates with pre-existing matrix, while in more developed constructs, nascent matrix lacks fibrous organization and is retained in the immediate pericellular space. Inhibition of collagen crosslinking increases matrix synthesis, but compromises matrix organization. Finally, these data demonstrate marked cell-to-cell heterogeneity amongst both chondrocytes and mesenchymal stem cells undergoing chondrogenesis. Collectively, these results introduce fluorescent noncanonical amino acid tagging as a strategy to investigate spatiotemporal matrix organization, and demonstrate its ability to identify differences in phenotype, microenvironment, and matrix assembly at the single cell level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.