ArticleScientific reports2016
RNA interference: a promising biopesticide strategy against the African Sweetpotato Weevil Cylas brunneus.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- Identification of broadly expressed nervous system-related genes as effective RNAi targets in the brown marmorated stink bug, Halyomorpha halys.Pest management science · 2026Article
- Development of a Droplet-Based RNA Interference Feeding Assay for Neonates of the Citrus Root Weevil Diaprepes abbreviatus.Archives of insect biochemistry and physiology · 2026Article
- Recent Advances in Sustainable Management ofInsects · 2026Review
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- Silencing ofInsects · 2022Article
- RNAi inFrontiers in plant science · 2022Article
- First Evidence of Feeding-Induced RNAi in Banana Weevil via Exogenous Application of dsRNA.Insects · 2021Article
- A new galling insect model enhances photosynthetic activity in an obligate holoparasitic plant.Scientific reports · 2021Article
- RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle.Communications biology · 2021Article
- Highly Variable Dietary RNAi Sensitivity Among Coleoptera.Frontiers in plant science · 2021Review
- Identification and functional analysis of five genes that encode distinct isoforms of protein phosphatase 1 in Nilaparvata lugens.Scientific reports · 2020Article
- Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae).Scientific reports · 2020Article
- RNAi-Mediated Suppression ofFrontiers in physiology · 2020Article
- Assessing the Risks of Topically Applied dsRNA-Based Products to Non-target Arthropods.Frontiers in plant science · 2020Article
- Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges.Frontiers in plant science · 2020Review
- Construction of Corynebacterium glutamicum cells as containers encapsulating dsRNA overexpressed for agricultural pest control.Applied microbiology and biotechnology · 2019Article
- Dietary Sugars Alter Hepatic Fatty Acid Oxidation via Transcriptional and Post-translational Modifications of Mitochondrial Proteins.Cell metabolism · 2019Article
- A nuclease specific to lepidopteran insects suppresses RNAi.The Journal of biological chemistry · 2018Article
- Implementing the sterile insect technique with RNA interference - a review.Entomologia experimentalis et applicata · 2017Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The African sweetpotato weevil Cylas brunneus is one of the most devastating pests affecting the production of sweetpotatoes, an important staple food in Sub-Saharan Africa. Current available control methods against this coleopteran pest are limited. In this study, we analyzed the potential of RNA interference as a novel crop protection strategy against this insect pest. First, the C. brunneus transcriptome was sequenced and RNAi functionality was confirmed by successfully silencing the laccase2 gene. Next, 24 potential target genes were chosen, based on their critical role in vital biological processes. A first screening via injection of gene-specific dsRNAs showed that the dsRNAs were highly toxic for C. brunneus. Injected doses of 200ng/mg body weight led to mortality rates of 90% or higher for 14 of the 24 tested genes after 14 days. The three best performing dsRNAs, targeting prosα2, rps13 and the homolog of Diabrotica virgifera snf7, were then used in further feeding trials to investigate RNAi by oral delivery. Different concentrations of dsRNAs mixed with artificial diet were tested and concentrations as low as 1 μg dsRNA/ mL diet led to significant mortality rates higher than 50%.These results proved that dsRNAs targeting essential genes show great potential to control C. brunneus.
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