ArticleGenes & development2016
The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts.
Article in Genes & development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 74 citations in OpenAlex.
- Spliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons.Nature communications · 2026Article
- Nuclear Argonaute:miRNA complexes recognize target sequences within chromatin-associated RNA and silence gene expression.Nucleic acids research · 2025Article
- Fission yeast Caprin protein is required for efficient heterochromatin establishment.PLoS genetics · 2025Article
- RNA binding by Periphilin plays an essential role in initiating silencing by the HUSH complex.Nucleic acids research · 2025Article
- PhpCNature communications · 2025Article
- RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.Cell · 2024Article
- Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Spt5 C-terminal repeat domain phosphorylation and length negatively regulate heterochromatin through distinct mechanisms.PLoS genetics · 2023Article
- The molecular basis of heterochromatin assembly and epigenetic inheritance.Molecular cell · 2023Review
- Recent Development in Biomedical Applications of Oligonucleotides with Triplex-Forming Ability.Polymers · 2023Review
- Broken up but still living together: how ARGONAUTE's retention of cleaved fragments explains its role during chromatin modification.Genes & development · 2023Review
- A universal model of RNA.DNA:DNA triplex formation accurately predicts genome-wide RNA-DNA interactions.Briefings in bioinformatics · 2022Article
- Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression.Molecular cell · 2022Article
- Review
- Separable roles for RNAi in regulation of transposable elements and viability in the fission yeast Schizosaccharomyces japonicus.PLoS genetics · 2022Article
- Hepatocellular carcinoma: Understanding molecular mechanisms for defining potential clinical modalities.World journal of hepatology · 2021Review
- Article
- Maternally inherited piRNAs direct transient heterochromatin formation at active transposons during earlyeLife · 2021Article
- Article
- An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing.PLoS genetics · 2021Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Small RNAs regulate chromatin modification and transcriptional gene silencing across the eukaryotic kingdom. Although these processes have been well studied, fundamental mechanistic aspects remain obscure. Specifically, it is unclear exactly how small RNA-loaded Argonaute protein complexes target chromatin to mediate silencing. Here, using fission yeast, we demonstrate that transcription of the target locus is essential for RNA-directed formation of heterochromatin. However, high transcriptional activity is inhibitory; thus, a transcriptional window exists that is optimal for silencing. We further found that pre-mRNA splicing is compatible with RNA-directed heterochromatin formation. However, the kinetics of pre-mRNA processing is critical. Introns close to the 5' end of a transcript that are rapidly spliced result in a bistable response whereby the target either remains euchromatic or becomes fully silenced. Together, our results discount siRNA-DNA base pairing in RNA-mediated heterochromatin formation, and the mechanistic insights further reveal guiding paradigms for the design of small RNA-directed chromatin silencing studies in multicellular organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.