ArticleProceedings of the National Academy of Sciences of the United States of America2016
Evolution of a mass spectrometry-grade protease with PTM-directed specificity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Multiomics analyses revealed the roles of the epithelial-mesenchymal transition and novel early candidate diagnostic biomarkers in neonatal necrotising enterocolitis.Clinical and translational medicine · 2026Article
- Citrullination in Tumor Metastasis, Inside and Outside the Cells.Cancer science · 2025Review
- Protease engineering: Approaches, tools, and emerging trends.Biotechnology advances · 2025Review
- Data-driven protease engineering by DNA-recording and epistasis-aware machine learning.Nature communications · 2025Article
- PERRC: Protease Engineering with Reactant Residence Time Control.ACS synthetic biology · 2025Article
- Leveraging yeast sequestration to study and engineer posttranslational modification enzymes.Biotechnology and bioengineering · 2024Review
- Characterizing citrullination by mass spectrometry-based proteomics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Review
- Hydrogel-Encapsulated Beads Enable Proximity-Driven Encoded Library Synthesis and Screening.ACS central science · 2023Article
- Mining proteomics data to extract post-translational modifications associated with gastric cancer.Amino acids · 2023Article
- ProtView: A Versatile Tool forJournal of proteome research · 2023Article
- Making the cut with protease engineering.Cell chemical biology · 2022Review
- Protein acetylation: a novel modus of obesity regulation.Journal of molecular medicine (Berlin, Germany) · 2021Review
- MrHAMER yields highly accurate single molecule viral sequences enabling analysis of intra-host evolution.Nucleic acids research · 2021Article
- Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity.Science (New York, N.Y.) · 2021Article
- Multiplexed Enzyme Activity-Based Probe Display via Hybridization.ACS combinatorial science · 2020Article
- Citrulline Not a Major Determinant in the Recognition of Peptidylarginine Deiminase 2 and 4 by Autoantibodies in Rheumatoid Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2020Article
- MS-based strategies for identification of protein SUMOylation modification.Electrophoresis · 2019Review
- Fluorescence-Activated Droplet Sorting for Single-Cell Directed Evolution.ACS synthetic biology · 2019Article
- Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.Nucleic acids research · 2018Article
- Advancement of analytical modes in a multichannel, microfluidic droplet-based sample chopper employing phase-locked detection.Analytical methods : advancing methods and applications · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mapping posttranslational modifications (PTMs), which diversely modulate biological functions, represents a significant analytical challenge. The centerpiece technology for PTM site identification, mass spectrometry (MS), requires proteolytic cleavage in the vicinity of a PTM to yield peptides for sequencing. This requirement catalyzed our efforts to evolve MS-grade mutant PTM-directed proteases. Citrulline, a PTM implicated in epigenetic and immunological function, made an ideal first target, because citrullination eliminates arginyl tryptic sites. Bead-displayed trypsin mutant genes were translated in droplets, the mutant proteases were challenged to cleave bead-bound fluorogenic probes of citrulline-dependent proteolysis, and the resultant beads (1.3 million) were screened. The most promising mutant efficiently catalyzed citrulline-dependent peptide bond cleavage (k
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.