ArticleProceedings of the National Academy of Sciences of the United States of America2016
Massive dysregulation of genes involved in cell signaling and placental development in cloned cattle conceptus and maternal endometrium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Beyond a single signal: How can bovine pregnancy be established without IFNT?The Journal of reproduction and development · 2026Review
- Bovine Endometrium Drives and Responds to Divergence of In Vitro Produced Conceptus Biochemistry.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Conceptus Elongation, Implantation, and Early Placental Development in Species with Central Implantation: Pigs, Sheep, and Cows.Biomolecules · 2025Review
- Transcription coactivator YAP1 promotes CCND1/CDK6 expression, stimulating cell proliferation in cloned cattle placentas.Zoological research · 2025Article
- Altered microRNA composition in the uterine lumen fluid in cattle (Bos taurus) pregnancies initiated by artificial insemination or transfer of an in vitro produced embryo.Journal of animal science and biotechnology · 2024Article
- Oviduct and endometrial epithelium improve in vitro produced bovine embryo developmental kinetics.Reproduction (Cambridge, England) · 2024Article
- Current status and future of gene engineering in livestock.BMB reports · 2024Review
- Extensive rewiring of the gene regulatory interactions between in vitro-produced conceptuses and endometrium during attachment.PNAS nexus · 2023Article
- Live births from urine derived cells.PloS one · 2023Article
- Cloning by SCNT: Integrating Technical and Biology-Driven Advances.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Ruminant conceptus-maternal interactions: interferon-tau and beyond.Journal of animal science · 2022Review
- Transcriptomic analysis of human endometrial stromal cells during early embryo invasion.Annals of medicine · 2021Article
- Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How.International journal of molecular sciences · 2020Review
- Rewiring of gene expression in circulating white blood cells is associated with pregnancy outcome in heifers (Bos taurus).Scientific reports · 2020Article
- Article
- Transcriptome-wide analysis of the SCNT bovine abnormal placenta during mid- to late gestation.Scientific reports · 2019Article
- Fine-tuned adaptation of embryo-endometrium pairs at implantation revealed by transcriptome analyses in Bos taurus.PLoS biology · 2019Article
- Interferon tau-dependent and independent effects of the bovine conceptus on the endometrial transcriptome†.Biology of reproduction · 2019Article
- Influences of sire conception rate on pregnancy establishment in dairy cattle.Biology of reproduction · 2018Article
- Silencing of retrotransposon-derived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
18 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major unresolved issue in the cloning of mammals by somatic cell nuclear transfer (SCNT) is the mechanism by which the process fails after embryos are transferred to the uterus of recipients before or during the implantation window. We investigated this problem by using RNA sequencing (RNA-seq) to compare the transcriptomes in cattle conceptuses produced by SCNT and artificial insemination (AI) at day (d) 18 (preimplantation) and d 34 (postimplantation) of gestation. In addition, endometrium was profiled to identify the communication pathways that might be affected by the presence of a cloned conceptus, ultimately leading to mortality before or during the implantation window. At d 18, the effects on the transcriptome associated with SCNT were massive, involving more than 5,000 differentially expressed genes (DEGs). Among them are 121 genes that have embryonic lethal phenotypes in mice, cause defects in trophoblast and placental development, and/or affect conceptus survival in mice. In endometria at d 18, <0.4% of expressed genes were affected by the presence of a cloned conceptus, whereas at d 34, ∼36% and <0.7% of genes were differentially expressed in intercaruncular and caruncular tissues, respectively. Functional analysis of DEGs in placental and endometrial tissues suggests a major disruption of signaling between the cloned conceptus and the endometrium, particularly the intercaruncular tissue. Our results support a "bottleneck" model for cloned conceptus survival during the periimplantation period determined by gene expression levels in extraembryonic tissues and the endometrial response to altered signaling from clones.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.