ArticleMolecular cell2016
The Mitotic Checkpoint Complex Requires an Evolutionary Conserved Cassette to Bind and Inhibit Active APC/C.
Article in Molecular cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- BubR1 TPR domain supports the mitotic checkpoint by promoting MCC formation and MCC-APC/C interaction.Communications biology · 2026Article
- Orchestrating homolog segregation in meiosis I: molecular logic and regulatory networks with emphasis on male metaphase I.Cell communication and signaling : CCS · 2026Review
- Article
- Principles and dynamics of spindle assembly checkpoint signalling.Nature reviews. Molecular cell biology · 2023Review
- The role of kinetochore dynein in checkpoint silencing is restricted to disassembly of the corona.Molecular biology of the cell · 2023Article
- Kinetochore-catalyzed MCC formation: A structural perspective.IUBMB life · 2023Article
- Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly.Nature communications · 2022Article
- Biochemical, biophysical, and functional characterisation of the E3 ubiquitin ligase APC/C regulator CDC20 fromFrontiers in physiology · 2022Article
- Counting Degrons: Lessons From Multivalent Substrates for Targeted Protein Degradation.Frontiers in physiology · 2022Review
- Spindle assembly checkpoint activation and silencing at kinetochores.Seminars in cell & developmental biology · 2021Review
- Molecular mechanism of Mad1 kinetochore targeting by phosphorylated Bub1.EMBO reports · 2021Article
- Coupling of Cdc20 inhibition and activation by BubR1.The Journal of cell biology · 2021Article
- Premature aging syndrome showing random chromosome number instabilities with CDC20 mutation.Aging cell · 2020Article
- Review
- A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase.EMBO reports · 2020Article
- Paradoxical mitotic exit induced by a small molecule inhibitor of APC/CNature chemical biology · 2020Article
- The Arabidopsis anaphase-promoting complex/cyclosome subunit 8 is required for male meiosis.The New phytologist · 2019Article
- Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C.Nature communications · 2019Article
- BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules.Cell research · 2019Article
- Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex.The EMBO journal · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The Spindle Assembly Checkpoint (SAC) ensures genomic stability by preventing sister chromatid separation until all chromosomes are attached to the spindle. It catalyzes the production of the Mitotic Checkpoint Complex (MCC), which inhibits Cdc20 to inactivate the Anaphase Promoting Complex/Cyclosome (APC/C). Here we show that two Cdc20-binding motifs in BubR1 of the recently identified ABBA motif class are crucial for the MCC to recognize active APC/C-Cdc20. Mutating these motifs eliminates MCC binding to the APC/C, thereby abolishing the SAC and preventing cells from arresting in response to microtubule poisons. These ABBA motifs flank a KEN box to form a cassette that is highly conserved through evolution, both in the arrangement and spacing of the ABBA-KEN-ABBA motifs, and association with the amino-terminal KEN box required to form the MCC. We propose that the ABBA-KEN-ABBA cassette holds the MCC onto the APC/C by binding the two Cdc20 molecules in the MCC-APC/C complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.