Evidence map›Paper›PMID 27939582›Full record

ArticleNeuron2016

New Transgenic Mouse Lines for Selectively Targeting Astrocytes and Studying Calcium Signals in Astrocyte Processes In Situ and In Vivo.

Rahul Srinivasan, Tsai-Yi Lu, Hua Chai, Ji Xu, Ben S Huang, Peyman Golshani, Giovanni Coppola, Baljit S Khakh

Open access · bronzeAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 309 papers.

0numbers the graph read from it
0cells of the map it votes in
309citing papers in PubMed
12.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

309 citing papers in PubMed, 463 citations in OpenAlex.

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  8. Astrocytes in Multiple Sclerosis: Getting to the Core.International journal of molecular sciences · 2026
    Review
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249 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Rahul SrinivasanDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Tsai-Yi LuDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Hua ChaiDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Ji XuDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Ben S HuangDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Peyman GolshaniDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Integrative Center for Learning and Memory, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; West Los Angeles VA Medical Center, Los Angeles, CA 90073, USA.
Giovanni CoppolaDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Center for Neurobehavioral Genetics, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Baljit S KhakhDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA. Electronic address: bkhakh@mednet.ucla.edu.
University of California, Los Angeles · US

Funding

Integrative Center for Neurogenetics and Neurogenomics - OverallP30NS062691 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ESKIN, ELEAZAR · 2009 to 2018
$7.4M
UCLA Center for Translational Research in Neurodevelopment: UC-TRaNU54HD087101 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MCCRACKEN, JAMES T. · 2016 to 2020
$6.5M
Astrocyte branchlet dysfunction as an early step in brain disordersDP1MH104069 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KHAKH, BALJIT · 2013 to 2017
$3.9M
New Optical and Genetic Tools to Study Diverse Calcium Signals in AstrocytesR01MH099559 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KHAKH, BALJIT · 2013 to 2017
$1.9M
Functional and Molecular Investigation of Physiological Astrocyte HeterogeneityF30MH106197 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHAI, HUA · 2015 to 2018
$162k
NICHD NIH HHS U54 HD087101NIMH NIH HHS DP1 MH104069NIMH NIH HHS F30 MH106197NIMH NIH HHS R01 MH099559NINDS NIH HHS P30 NS062691
6 · The paper itself

Abstract

Astrocytes exist throughout the nervous system and are proposed to affect neural circuits and behavior. However, studying astrocytes has proven difficult because of the lack of tools permitting astrocyte-selective genetic manipulations. Here, we report the generation of Aldh1l1-Cre/ERT2 transgenic mice to selectively target astrocytes in vivo. We characterized Aldh1l1-Cre/ERT2 mice using imaging, immunohistochemistry, AAV-FLEX-GFP microinjections, and crosses to RiboTag, Ai95, and new Cre-dependent membrane-tethered Lck-GCaMP6f knockin mice that we also generated. Two to three weeks after tamoxifen induction, Aldh1l1-Cre/ERT2 selectively targeted essentially all adult (P80) brain astrocytes with no detectable neuronal contamination, resulting in expression of cytosolic and Lck-GCaMP6f, and permitting subcellular astrocyte calcium imaging during startle responses in vivo. Crosses with RiboTag mice allowed sequencing of actively translated mRNAs and determination of the adult cortical astrocyte transcriptome. Thus, we provide well-characterized, easy-to-use resources with which to selectively study astrocytes in situ and in vivo in multiple experimental scenarios.

Indexed as

Calcium SignalingAldehyde DehydrogenaseAnimalsAstrocytesCalciumCytosolEstrogen Receptor betaGABA Plasma Membrane Transport ProteinsGene Expression ProfilingIntegrasesMiceMice, TransgenicModels, AnimalRNA, MessengerSelective Estrogen Receptor ModulatorsTamoxifenAldehyde DehydrogenaseCalciumCre recombinaseEstrogen Receptor betaGABA Plasma Membrane Transport ProteinsIntegrasesRNA, MessengerSelective Estrogen Receptor ModulatorsSlc6a11 protein, mouseTamoxifenAldh1l1astrocytecalciumCre/ERT2GCaMPhippocampusLck-GCaMPstriatumvisual cortex

Identifiers

PMID27939582
PMCPMC5403514
OpenAlexW2559768163

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.