ArticleThe Journal of cell biology1989
Formation of acetylcholine receptor clusters in chick myotubes: migration or new insertion?
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Increased ratio of rapsyn to ACh receptor stabilizes postsynaptic receptors at the mouse neuromuscular synapse.The Journal of physiology · 2005Article
- Weak dependence of mobility of membrane protein aggregates on aggregate size supports a viscous model of retardation of diffusion.Biophysical journal · 1999Article
- Intercellular communication that mediates formation of the neuromuscular junction.Molecular neurobiology · 1997Review
- Common molecular mechanisms in field- and agrin-induced acetylcholine receptor clustering.Cellular and molecular neurobiology · 1997Article
- Immobilization of nicotinic acetylcholine receptors in mouse C2 myotubes by agrin-induced protein tyrosine phosphorylation.The Journal of cell biology · 1995Article
- ARIA is concentrated in the synaptic basal lamina of the developing chick neuromuscular junction.The Journal of cell biology · 1995Article
- Density and diffusion limited aggregation in membranes.Bulletin of mathematical biology · 1995Article
- Developmental regulation of membrane traffic organization during synaptogenesis in mouse diaphragm muscle.The Journal of cell biology · 1995Article
- Compartmentalized structure of the plasma membrane for receptor movements as revealed by a nanometer-level motion analysis.The Journal of cell biology · 1994Article
- Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells.Biophysical journal · 1993Article
- Clustering and immobilization of acetylcholine receptors by the 43-kD protein: a possible role for dystrophin-related protein.The Journal of cell biology · 1993Article
- Extracellular synaptic factors induce clustering of acetylcholine receptors stably expressed in fibroblasts.The Journal of cell biology · 1991Article
- Single particle tracking. Analysis of diffusion and flow in two-dimensional systems.Biophysical journal · 1991Article
- Acetylcholine receptor-inducing activity stimulates expression of the epsilon-subunit gene of the muscle acetylcholine receptor.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Differences between the lateral organization of conventional and inositol phospholipid-anchored membrane proteins. A further definition of micrometer scale membrane domains.The Journal of cell biology · 1991Article
- FRAP, FLIM, and FRET: Detection and analysis of cellular dynamics on a molecular scale using fluorescence microscopy.Molecular reproduction and developmentReview
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Experiments were performed to study the feasibility of two mechanisms of acetylcholine receptor (ACHR) accumulation in chick myotubes: diffusion and trapping of previously dispersed surface receptors and localized insertion of new receptors at accumulation sites. Fluorescence photobleaching recovery (FPR) measurements indicated that the majority of diffusely distributed ACHRs in chick myotube membranes were mobile whereas nearly all receptors within high density clusters were effectively immobile. Unlike previous reports, two rates of ACHR movement characterized the mobile population. Moreover, we found that the estimated diffusion coefficient depended critically on the objective (spot size) used to assay recovery from bleaching. Implications of this finding for mechanisms of receptor immobilization are discussed. Extracts of chick brain, known to increase the number of surface receptors, did not alter receptor mobility. Extracts of Torpedo electric organ that increase the number of receptor aggregates, decreased the mobile fraction of ACHRs. Simulations of the diffusion and trapping mechanism indicated that captured receptors should congregate around the periphery of a receptor patch during the first hour after they were inserted into the membrane. However, newly inserted ACHRs were found to be located centrally within receptor patches under neurites, and this was not consistent with an exclusive diffusion-trapping mechanism. We also studied the mobility of ACHRs near points of contact made by cholinergic growth cones. The rate of receptor movement was increased in the vicinity of growth cones, but the magnitude of this effect was small.
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